为有效利用废弃香蕉皮资源并提高产品附加值，该研究采用乳酸菌发酵辅助酶法制备香蕉皮膳食纤维（BP-SDF）并优化其工艺条件，进一步分析了最佳工艺下香蕉皮粉（BP）、BP-SDF及香蕉皮不溶性膳食纤维（BP-IDF）的理化及功能性质。结果表明：最佳发酵工艺为鼠李糖乳杆菌接种量为3.0%、发酵温度38℃、发酵时间18 h，BP-SDF得率为32.18%±0.82%，鼠李糖乳杆菌发酵辅助酶法对BP-SDF提取率较传统酶法提高了2倍。与酶法提取香蕉皮膳食纤维相比，BP-SDF的溶解性、持水力及吸水膨胀力均极显著提升，溶解性增强7.1倍，持油力变化不明显。BP-SDF对DPPH、O2-·、ABTS、OH·自由基具有一定清除能力，表现出较好的抗氧化活性。BP-SDF和BP-IDF对胆固醇、亚硝酸盐吸附能力均显著提升，且两者在胃环境（pH=2）中吸附能力显著高于肠道环境（pH=7），此外BP-SDF还表现出良好的降血糖活性。因此，乳酸菌发酵辅助酶法是一种可行的香蕉皮皮膳食纤维制备方法，可为香蕉皮粉膳食纤维功能研究提供理论依据。

In order to effectively utilize waste banana peel resources and increase the added value of products, the Lactobacillus fermentation-assisted enzymatic method was used to extract the banana peel dietary fibers. The fermentation process conditions were optimized and its physicochemical properties and functional characteristics of banana peel powder (BP), banana peel soluble dietary fiber (BP-SDF) and banana peel insoluble dietary fiber (BP-IDF) were analyzed. The results showed that the optimal fermentation process was as follows: 3.0% Lactobacillus rhamnosus inoculation, fermentation temperature 38℃, fermentation time 18h, Under this condhions, the yield of BP-SDF was 32.18%±0.82%, the extraction rate of BP-SDF was 2 times higher than that by traditional enzyme method. Compared with enzymatic extraction of dietary fibers from banana peel, the solubility, water holding capacity and water absorption expansion capacity of BP-SDF were significantly improved, the solubility was increased by 7.1 times, but the oil holding capacity was not significantly changed. In addition, BP-SDF could scavenge DPPH, O2-·, ABTS and OH·free radicals, demonstrating good antioxidant activity. The adsorption capacity of BP-SDF and BP-IDF on cholesterol and nitrite was significantly improved., and the adsorption capacity of BP-SDF in stomach environment (pH=2) was significantly higher than that in intestinal environment (pH=7). Besides, BP-SDF showed better hypoglycemic activity.Therefore, this study demonstrates the Lactobacillus fermentation assisted enzyme method was a feasible method to prepare dietary fiber of banana peel, meanwhile this study will provide theoretical basis for the high-value utilization rate of banana peel dietary fibers.

2023年度广西高校中青年教师科研基础能力提升项目(2023KY0764；2023KY0765；)；2024年度广西高校中青年教师科研基础能力提升项目(2024KY0756)；2024年第一批百色市科学研究与技术开发计划自筹经费项目(百科20241550)；2022年百色市科学研究与技术开发计划项目(百科20222009)；广西一流学科(农业资源与环境)(No.1Guijiaokeyuan[2022])。