该文研究蝉棒束孢（Isaria cidada Miquel）C1菌丝体多糖ISP-80对脂多糖（LPS）诱导的小鼠单核巨噬细胞RAW264.7炎症模型的抗炎效果，并探讨其作用机制。以热水浸提法获得菌丝体多糖ISP-80，采用CCK-8法测定ISP-80对RAW264.7细胞的毒性作用，使用ELISA试剂盒测定TNF-α、IL-1β、IL-6细胞因子分泌量，通过Western Blot法检测MAPK/NF-κB信号通路中相关蛋白的含量。结果显示，ISP-80多糖提取率为10.42%，共含有3种多糖组分，由葡萄糖（Glc）、甘露糖（Man）、半乳糖（Gal）3种单糖组成，摩尔比为1.00:5.03:1.55。ISP-80多糖在添加0.01~1.00 mg/mL质量浓度范围内时对细胞无毒性且能促进细胞增殖（P<0.000 1）。0.25 mg/mL ISP-80处理组可显著降低促炎细胞因子IL-1β（P<0.001）和IL-6（P<0.05）的分泌，分别降至LPS组的0.59倍和0.88倍，且可明显抑制细胞分化，恢复细胞形态。ISP-80的抗炎作用是通过MAPK和NF-κB途径介导的，ISP-80通过阻断p-p38和p-JNK来抑制MAPK途径，分别降低了0.20和0.41（P<0.001），并且通过阻断IκBα和p65来抑制NF-κB通路，分别降低了0.43（P<0.01）和0.24（P<0.001）。因此，ISP-80具有较好的抗炎作用，可作为预防和治疗炎症性疾病的天然和安全的抗炎剂。

The anti-inflammatory effects and mechanisms of the mycelial polysaccharide ISP-80 from the Isaria cicada Miquel C1 was investigated in a mouse monocyte-macrophage RAW264.7 inflammation model, induced by lipopolysaccharide (LPS). The polysaccharide (ISP-80) was obtained through hot water extraction from the Isaria cidada C1 mycelium. The toxic effects of ISP-80 on RAW264.7 cells were evaluated using the CCK-8 assay, and the impact of ISP-80 on the secretion of TNF-α, IL-1β, and IL-6 cytokines was measured using ELISA. Furthermore, the mechanism of ISP-80 was analyzed using western blotting to detect the relevant proteins in the MAPK/NF-κB pathways. The findings indicated that the extraction rate of ISP-80 was 10.42% and it was composed of three polysaccharide fractions, each containing Glc, Man, and Gal monosaccharides, in a molar ratio of 1.00:5.03:1.55. Adding ISP-80 at 0.01~1.00 mg/mL exerted no cytotoxic effects and promoted cell proliferation (P<0.000 1). The 0.25 mg/mL ISP-80 treatment significantly decreased the secretion of pro-inflammatory cytokines IL-1β (P<0.001) and IL-6 (P<0.05), reaching 0.59- and 0.88-times those of the LPS group, respectively, and inhibited cell differentiation while restoring cell morphology. The anti-inflammatory action of ISP-80 acted via the MAPK/NF-κB pathways. ISP-80 inhibited the MAPK pathway by blocking p-p38 and p-JNK, which decreased by 0.20 and 0.41, respectively (P<0.001). Furthermore, the NF-κB pathway was inhibited by blocking IκBα and p65, which decreased by 0.43 (P<0.01) and 0.24 (P<0.001), respectively. These results indicate that ISP-80 can be a natural and safe antiinflammatory agent for the prevention and treatment of inflammatory diseases.

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