为研究韭黄提取物不同萃取部位的抗肿瘤活性及化学组成，运用MTT法、划痕实验分别检测不同萃取部位提取物对A549细胞增值和迁移的影响，流式细胞术检测活性部位（HC-Ea）处理后细胞周期分布情况，Western Blot检测HC-Ea作用后A549细胞中周期相关蛋白CDK2、CDK4、CDC25A、Cyclin A2的表达；超高液相色谱-四极杆-静电场轨道阱高分辨质谱技术（UPLC-Q-Orbitrap HR/MS）分析HC-Ea的化学成分。A549细胞经萃取部位处理后抑制率随浓度增加而增加，HC-Ea在24、48及72 h的抑制质量浓度（IC50 值）分别为50.81、32.09和25.02 μg/mL。与DMSO组相比，50、100及200 μg/mL的HC-Ea剂量组均能抑制划痕愈合率，抑制作用随药物浓度增加而增强。A549细胞经HC-Ea处理48或72 h，各浓度组细胞S期比例显著升高，且呈剂量依赖关系。与DMSO组相比，HC-Ea各浓度组A549细胞中CDK2、CDK4、CDC25A、Cyclin A2蛋白表达降低，其中高浓度组表现尤为突出。UPLC-Q-Orbitrap HR/MS从韭黄提取物中检出25个化合物，其中含氮化合物10个、有机酸类8个。韭黄抗肿瘤活性与物质组成的研究为韭黄产业化应用提供了新的借鉴。

The antitumor activity and chemical composition of extracts from different Allium senescens tissues are presented in this study. MTT assays and scratch tests were used to detect the effects of different plant tissue extracts on the A549 cell proliferation and migration. Cell cycle distribution in control and treated cells was detected by flow cytometry and the expression of cycle-related proteins (CDK2, CDK4, CDC25A, and Cyclin A2) in A549 cells treated with HC-Ea were measured by western blot. The chemical composition of each HC-Ea was analyzed by UPLC-Q-Orbitrap HR/MS. Inhibition of A549 cell proliferation increased with increasing concentration, with respective HC-Ea inhibitory concentrations (IC50 values) of 50.81, 32.09 and 25.02 μg/mL observed at 24, 48, and 72 h, respectively. Compared with DMSO, 50, 100, and 200 μg/mL doses of each HC-Ea reduced the scratch healing rate, with their inhibitory effects intensifying with increasing drug dosage. Following treatment of A549 cells with HC-Ea for 48 or 72 h, the proportion of S phase cells in each concentration group increased significantly in a dose-dependent manner. Compared with DMSO treated cells, the expression of CDK2, CDK4, CDC25A, and Cyclin A2 protein in A549 cells in each HC-Ea treated group was reduced, especially with high concentration exposure. A total of 25 compounds from Allium senescens extract were detected by UPLC-Q-Orbitrap HR/MS, including 10 nitrogen compounds and 8 organic acids. This research on the antitumor activity and chemical composition of Allium senescens provides a new reference for industrial applications.

贵州省省级科技计划项目资助（黔科合支撑[2021]一般110）