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[摘要]
为分析发酵乳杆菌(L. fermentum)菌株在发酵南酸枣汁时的有机酸降解及其产香特征。采用高效液相色谱和气相色谱-质谱联用技术测定乳酸菌发酵前、后南酸枣汁有机酸和主要香气物质变化。经过L. fermentum菌株的发酵处理,南酸枣汁中的柠檬酸含量显著降低,从4.91 g/L降至0.90 g/L,苹果酸含量从4.94 g/L降至4.61 g/L,同时,发酵过程中产生了较多的乳酸(约为6.18 g/L)以及少量的乙酸(约为 0.18 g/L)。L. fermentum菌株发酵过程中产生的乙酸乙酯、2-辛酮、苯甲酸乙酯、芳樟醇和香叶醇等香气物质增强了南酸枣汁青草、柑橘和玫瑰等花果香气,使得发酵后的南酸枣汁的香气层次丰富、柔和,通过多元统计分析方法确定了6种L. fermentum菌株发酵南酸枣汁的差异风味化合物。由此可见,L. fermentum菌株在发酵南酸枣汁时不仅能够降解柠檬酸和苹果酸,还表现出良好的产香能力,为乳酸菌发酵果汁的品质提升和新产品的开发提供了重要思路。
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[Abstract]
To analyze the degradation of organic acids and aroma-producing characteristics of Lactobacillus fermentum strains during the fermentation of Choerospondias axillaris juice, high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) techniques were employed to measure the changes in organic acids and major aroma compounds in the juice before and after lactic acid bacteria fermentation. After being fermented by L. fermentum strains, the citrate content in Choerospondias axillaris juice was significantly reduced from 4.91 g/Lto 0.90 g/L, and the malate content decreased from 4.94 g/L to 4.61 g/L. Simultaneously, a considerable amount of lactic acid (approximately 6.18 g/L) and a small quantity of acetic acid (around 0.18 g/L) were produced during the fermentation process. Aroma compounds such as ethyl acetate, 2-octanone, ethyl benzoate, linalool, and geraniol, generated during the fermentation of L. fermentum strains, enhanced the grassy, citrusy, and rosy floral-fruit aromas of Choerospondias axillaris juice, resulting in a rich and mellow aroma profile after fermentation. Six differential flavor compounds in Choerospondias axillaris juice fermented by L. fermentum strains were identified through multivariate statistical analysis. It can be concluded that during the fermentation of Choerospondias axillaris juice, L. fermentum strains not only degraded citrate and malate but also exhibited good aroma-producing capabilities, providing significant insights for improving the quality of lactic acid bacteria-fermented fruit juices and developing new products.
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