白番茄提取物组合物抗糖化及抗小鼠衰老作用评价

Anti-glycation Effects of Lumenato Extract Complex and Its Anti-aging Effects in Mice

摘要:

该研究通过建立体外抗糖化模型、抗氧化模型及小鼠衰老模型，评价白番茄组合物抗糖化、抗氧化及抗小鼠衰老的效果。实验结果表明，在质量浓度为0.2 mg/mL时，组合物对果糖胺、活性羰基化合物和荧光性晚期糖基化终末产物抑制率分别为44.39%、19.22%和69.28%，组合物抗糖基化效果优于氨基胍。组合物对O2-•、羟基和ABTS+自由基均有显著的清除作用，组合物的清除自由基能力：ABTS+•>•OH>O2-•。细胞实验表明，组合物可显著减轻丙酮醛的细胞毒性，提高HepG2细胞活力（P<0.05）。在小鼠衰老模型中，组合物可改善D-半乳糖诱导的肝损伤并显著恢复肝脏总超氧化物歧化酶水平（P<0.05）。由此可知，这款含白番茄提取物组合物具有较好的抗糖基化、抗氧化能力和抗衰老能力，将在功能食品、保健品行业有较好的应用前景。

Abstract:

The anti-glycation, antioxidant, and anti-aging effects of Lumenato extract complex were examined by establishing in vitro anti-glycation and antioxidant models and an in vivo model of aging in mice, respectively. The results revealed that the application of the extract complex at a concentration of 0.2 mg/mL resulted in inhibition rates of 44.39%, 19.22%, and 69.28% were for fructosamine, active carbonyl compounds, and fluorescent advanced glycation end-products, respectively. Furthermore, the anti-glycation effects of the complex were found to be superior to those of aminoguanidine, and the complex exhibited significant scavenging effects on O2-•, OH, and ABTS+• (in the order ABTS+•>•OH>O2-•). In addition, cell experiments revealed that the complex can significantly reduce the cytotoxicity of methylglyoxal and enhance the viability of HepG2 cells (P<0.05). In the mouse aging model, the complex alleviated the liver injury induced by D-galactose, as well as restored liver total superoxide dismutase levels (P<0.05). These findings thus provide evidence indicating that with respect to its anti-glycation, antioxidant, and anti-aging properties, Lumenato extract complex has promising application potential in the functional food and health product industries.