该文研究了荔枝发酵物对人体皮肤HaCat 细胞模型中的ROS、SOD、透明质酸和水通道蛋白AQP3生成，以及对细胞周期与细胞凋亡的影响。结果表明，在双氧水诱导的HaCat细胞模型中，中剂量和高剂量组的ROS生成抑制率达到50%以上；所有剂量组均显著提升SOD活力水平（P<0.05），高剂量组相比模型组提高39.96%，与空白对照组水平一致（P>0.05）。在HaCat细胞干燥模型中，所有剂量组荔枝发酵物均显著提升水通道蛋白AQP3和透明质酸的表达水平，高剂量组相比模型组分别提高2.08倍和1.11倍；荔枝发酵物能够调节HaCat细胞由G0/G1期比例向S期和G2/M期转化（P<0.01），且能够显著降低由UVA照射导致的HaCat人体皮肤细胞的凋亡率（P<0.05）。因此，荔枝发酵物能有效提升HaCat细胞的抗氧化能力，促进细胞分裂，修复UVA导致的细胞凋亡，为其在皮肤健康产品研究和应用提供了参考。

The effects of lychee fermentation products on ROS, SOD, hyaluronic acid, and aquaporin 3 (AQP3) production in human HaCaT cells as well as on the cell cycle and apoptosis were examined. In HaCaT cell models with hydrogen peroxide-induced oxidative damage, medium and high doses of lychee fermentation products suppressed the ROS production rate by more than 50%. All groups treated with lychee fermentation products showed significantly higher SOD activity levels, regardless of the treatment dose (P<0.05). The SOD activity level of the high-dose treatment group was 39.96% higher than that of the model group and comparable to that of the control group (P>0.05). In the HaCaT cell drying models, lychee fermentation products at all doses increased the expression levels of AQP3 and hyaluronic acid; specifically, the high-dose treatment group exhibited levels 2.08 and 1.11 times higher, respectively, compared to the model group. Furthermore, lychee fermentation products could regulate the progression of HaCaT cells from the G0/G1 phase to the S and G2/M phases (P<0.01) and significantly reduced the UVA irradiation-induced rate of cell apoptosis (P<0.05). Thus, lychee fermentation products can effectively enhance the antioxidative capability of HaCaT cells, promoting their division while preventing their apoptosis. These findings provide valuable reference data for research on the application of lychee fermentation products in the development of skin care products.

华南协同研究院产学研项目（2021440002001713）