该实验旨在探究鸡胆汁酶转化物（CB）对大鼠酒精性肝病（ALD）的保护作用，并揭示其作用机制。35只8周龄的雄性大鼠适应性喂养一周后，随机分为正常组、模型组、CB低中高剂量（50、100、150 mg/kg）。经过一周6 mL/kg•bw的酒精造模及CB干预后处死大鼠收集血清及肝脏测定相关指标，通过H&E 染色观察肝组织形态。结果表明，高剂量CB具有良好的护肝效果，改善了大鼠体质量的降低及肝脏指数的升高。高剂量CB干预后，肝脏中乙醇脱氢酶（ADH）、乙醛脱氢酶（ALDH）和过氧化氢酶（CAT）活性分别提高67.48%、110.07%、34.30%，细胞色素酶（CYP2E1）水平降低35.02%，酒精代谢能力显著提高，同时，血清中谷草转氨酶（AST）、碱性磷酸酶（ALP）和谷丙转氨酶（ALT）活性分别降低35.62%、38.50%、45.25%，改善肝脏损伤。另外，高剂量CB干预可降低血清中51.23%总胆固醇（TC）和47.12%甘油三酯（TG）的水平，提高脂质代谢，同时，肝脏中超氧化物歧化酶（SOD）活力提高102.93%，谷胱甘肽（GSH）水平提高40.69%，丙二醛（MDA）水平降低35.85%，减缓肝脏氧化应激。高剂量CB干预可下调TLR4/MyD88/NF-κB/IKBα信号通路的基因和蛋白的表达，且白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、白细胞介素8（IL-8）和肿瘤坏死因子α（TNF-α）水平分别降低31.29%、28.71%、40.48%和37.48%，减缓炎症应激。研究结果表明，CB具有开发成为护肝功能性食品及保健品的潜力。

The protective effects of enzymatically converted chicken bile (CB) on alcoholic liver diseases (ALD) in rats and its potential mechanism were investigated. Thirty-five 8-week-old male rats were randomly divided into a control group; a model group; and low-, medium- and high-dose CB treatment groups (50, 100, and 150 mg/kg, respectively) after adaptive feeding for 1 week. At 1 week after modeling using 6 mL/kg•bw of alcohol and CB intervention, all rats were sacrificed. Serum and liver samples were collected to determine relevant indices, and the liver histology was observed using hematoxylin and eosin staining. The results showed that a high dose of CB was associated with hepatoprotective effects by ameliorating the reduction in body weight and elevated liver index in rats. After high-dose CB intervention, the activities of hepatic alcohol dehydrogenase, acetaldehyde dehydrogenase, and catalase were increased by 67.48%, 110.07%, and 34.30%, respectively, whereas the cytochrome enzyme level decreased by 35.02%, indicating a substantial enhancement in alcohol metabolism. Moreover, the activities of alanine transaminase, alkaline phosphatase, and alanine transaminase in the serum were reduced by 35.62%, 38.50%, and 45.25% respectively, indicating improvements in liver damage. In addition, CB intervention reduces the levels of total cholesterol (by 51.23%) and triglycerides (by 47.12%) in the serum, increased superoxide dismutase activity (by 102.93%) and the glutathione level (by 40.69%), and decreased the malondialdehyde level (by 35.85%) in the liver. These results suggest that lipid metabolism was enhanced and oxidative stress was alleviated. Highdose CB intervention downregulates the expression levels of genes and proteins involved in the TLR4/MyD88/NF-κB/IKBα signaling pathway. The expression levels of interleukin 1β, interleukin 6, interleukin 8, and tumor necrosis factor α were reduced by 31.29%, 28.71%, 40.48%, and 37.48%, respectively, indicating reduced inflammatory stress. Based on these findings, CB shows potential for development as a functional food and health care product for liver protection.

财政部和农业农村部：国家现代农业产业技术体系（CARS-21）