探讨长链菊粉对小鼠湿疹的改善作用。采用2,4-二硝基氯苯（DNCB）构建小鼠湿疹模型，给予长链菊粉添加量分别为5%和20%的饲料，持续干预3周后检测小鼠皮肤和结肠组织形态变化、肠道菌群组成、脾脏和胸腺的氧化应激及炎症因子水平。长链菊粉可显著改善湿疹小鼠皮肤角化、棘层增厚等症状，减轻肠道黏膜损伤和炎症反应。长链菊粉干预降低了葡萄球菌属和瘤胃球菌属的相对丰度，增加了双歧杆菌属、乳杆菌属和阿克曼氏菌属的相对丰度。此外，与模型组相比，长链菊粉低、高剂量组胸腺的总超氧化物歧化酶（T-SOD）活性和白细胞介素-10（IL-10）水平显著升高，而丙二醛（MDA）含量及白细胞介素-6（IL-6）水平明显下降。长链菊粉低剂量组脾脏中T-SOD活性显著升高而MDA含量显著降低，长链菊粉高剂量组IL-10水平明显升高而IL-6水平明显下降。综上，长链菊粉能改善DNCB诱导的小鼠湿疹，对湿疹小鼠肠道菌群的组成、免疫器官的氧化损伤及炎症反应有一定的调节作用。

Improving the effects of long-chain inulin on eczema in mice was investigated using a model of eczema induced with 2,4-dinitrochlorobenzene and feeding mice a diet containing 5% or 20% long-chain inulin for 3 weeks. The effects of long-chain inulin on eczema were evaluated based on pathological changes in skin and colon tissues, fecal microbiota, oxidative stress status, and inflammation-related factors of the thymus and spleen. The results revealed that the symptoms of skin keratosis and spinous layer thickening were significantly alleviated in both low- and high-dose longchain inulin intervention groups, along with significant reductions in intestinal mucosal injury and inflammatory responses. The results of 16S rRNA sequencing indicated reductions in the relative abundance of Staphylococcus and Ruminococcus in the low- and high-dose intervention groups, whereas there were increases in the relative abundance of Bifidobacterium, Lactobacillus, and Akkermansia. Furthermore, compared with the model group, low- and high-dose inulin intervention contributed to significant increases in the activity of T-SOD and the levels of IL-10 in the thymus, along with significant reductions in the contents of MDA and levels of IL-6. Similarly, compared with the model group, the spleens of mice in the low-dose inulin intervention group showed significant increases in T-SOD activity and reductions in MDA levels, whereas high-dose inulin intervention promoted significant increases and reductions in IL-10 and IL-6 levels, respectively, in spleens. These results indicate that long-chain inulin has the potential to ameliorate 2,4-dinitrochlorobenzene-induced eczema by regulating the gut microbiota, reducing oxidative stress, and attenuating inflammatory reactions.

广西自然科学基金资助项目（2021GXNSFBA075038）；大学生创新创业训练计划项目（202010601033；S202210601147）