为了探究山苍籽精油对分离自婴儿配方乳粉的野生株阪崎克罗诺杆菌的抑制作用，采用连续二倍稀释法、平板计数法和生长曲线评价山苍籽精油对阪崎克罗诺杆菌的抑菌活性，通过检测β-半乳糖苷酶活性、碱性磷酸酶活性、胞内DNA含量变化及细菌结构变化等指标探究其抑菌机制。结果表明，山苍籽精油对阪崎克罗诺杆菌具有较强的抑菌活性，其对阪崎克罗诺杆菌的MIC为6.62 μg/mL。经1 MIC的山苍籽精油处理后阪崎克罗诺杆菌的生长明显受到抑制，胞外碱性磷酸酶和胞内β-半乳糖苷酶活性显著增加为对照组的2倍和13.50倍（P<0.05）；与对照组相比，菌体的细胞形态遭到破坏，细胞内容物泄露，核酸和蛋白质泄露量皆是对照组的1.03倍（P<0.05），胞内DNA含量降低至0.17 μg/mL，DAPI荧光区域降低以及凝胶阻滞电泳条带亮度变暗，DNA受损。综上所述，山苍籽精油可作为阪崎克罗诺杆菌的高效抑菌剂，通过破坏细胞膜和DNA，干扰菌体正常生理活性进而导致菌体死亡。该研究为开发山苍籽精油为新型抑菌剂提供了理论基础。

To investigate the antibacterial effect of Litsea cubeba oil on wild strains of Cronobacter sakazakii isolated from infant formula milk powder, the antibacterial activity of Litsea cubeba oil against Cronobacter sakazakii was evaluated by continuous double dilution method, plate counting method and growth curve. The antibacterial mechanism was investigated by detecting β-galactosidase activity, alkaline phosphatase activity, intracellular DNA content and bacterial structure changes. The results showed that Litsea cubeba oil had strong antibacterial effect against Cronobacter sakazakii. The MIC of Litsea cubeba oil against Cronobacter sakazakii was 6.62 μg/mL. The growth of Cronobacter sakazakii was significantly inhibited after 1 MIC treatment, and the activity of extracellular alkaline phosphatase and intracellular β-galactosidase in Cronobacter sakazakii were significantly increased by 2 times and 13.50 times as compared with the control group(P < 0.05). Compared with the control group, the cell morphology of the bacteria was destroyed, the contents of the cell were leaked, the amount of nucleic acid and protein leaked was 1.03 times that of the control group (P<0.05), the intracellular DNA content was reduced to 0.17 μg/mL and DAPI fluorescence area was decreased, and the brightness of the gel block electrophoresis bands was darkened, and DNA was damaged. In summary, the essential oil of Litsea cubeba oil could be used as a highly effective bacteriostatic agent for Cronobacter sakazakii by destroying cell membrane and DNA, and interfering with the normal physiological activity of bacteria, and leaded to the death of bacteria. This study provided a theoretical basis for developing the essential oil of Litsea cubeba oil as a new antibacterial agent.