旨在探究6-姜烯酚对衰老小鼠氧化应激的影响。采用腹腔注射D-半乳糖（D-galactose, D-gal）建立衰老小鼠模型，同时用槲皮素，低、中、高剂量（按体质量计6、12、18 mg/kg）6-姜烯酚给小鼠灌胃。记录小鼠脏器与体质量比值；苏木精-伊红（Hematoxylin Eosin, HE）染色检测组织病理学变化；β-半乳糖苷酶（Senescence-Associated β-galactosidase, SA-β-Gal）染色法测定组织器官衰老情况；检测组织中总超氧化物歧化酶（Superoxide Dismutase，SOD）、谷胱甘肽过氧化物酶（Glutathione Peroxidase，GSH-Px）和丙二醛（Malondialdehyde，MDA）含量。结果显示，与衰老模型组相比，中剂量组脏器系数升高最多（P<0.05）。病理组织切片显示，经6-姜烯酚干预后，小鼠心肌细胞坏死减少，肾小体形态趋于正常，生精小管萎缩情况得到改善，精子数量增多。SA-β-Gal染色结果显示，与衰老模型组相比，中剂量6-姜烯酚组小鼠各组织器官切片蓝染较轻，器官衰老程度有明显改善。与衰老模型组相比，不同浓度的6-姜烯酚组小鼠心脏、肾脏及睾丸中抗氧化酶活力升高、过氧化产物水平下降。其中，中剂量6-姜烯酚组的SOD和GSH-Px的活性显著升高（P<0.05），MDA 含量显著降低（P<0.05）。上述结果表明，6-姜烯酚改善衰老小鼠相关脏器的损伤与氧化应激有关。

To investigate the effects of 6-shogaol on oxidative stress in aging mice, a model was established by injecting D-galactose into the abdomen of aging mice. Quercetin and low, medium and high doses of 6-shogaol (6, 12 and 18 mg/kg by body mass, respectively) were administered to mice by gavage. The organ coefficients of the heart, kidneys, and testes was recorded, and hematoxylin-eosin staining was used to detect histopathological changes. β-Galactosidase (SA-β-Gal) staining was used to determine the degree of aging of the organs. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and the malondialdehyde (MDA) content of mouse tissues were measured. The organ coefficients were highest in the medium-dose group and significantly higher than those in the aging model group (P<0.05). Pathological sections showed that after 6-shogaol intervention, cardiomyocyte necrosis decreased, the number of renal corpuscles tended to normalize, seminiferous tubule atrophy was improved, and the number of sperm increased. Various tissue and organ sections in the medium-dose 6-shogaol group showed lighter SA-β-Gal staining, and the degree of organ aging was significantly improved compared with the aging model group. Antioxidant enzyme activity was elevated and peroxidation product levels were decreased in the heart, kidneys, and testes of mice in the different 6-shogaol treatment groups than in the aging model group. The activities of SOD and GSH-Px were significantly higher (P<0.05) and the MDA content was significantly lower (P<0.05) in the medium-dose 6-shogaol group than in the aging model group. The findings of this study suggest that 6-shogaol ameliorates oxidative-stress-induced damage to important organs in aging mice.

广西科技重大专项（AA22096026）；广西研究生教育创新计划项目（GYYK2022002）；桂林市创新平台和人才计划（20220120-2）