为了提高益生菌递对到胃肠道强酸、消化酶、胆盐等耐受性，本研究将纳米纤维素（Cellulose Nanofibril，CN）与海藻酸钠（Sodium Alginate，SA）进行复配，分别通过傅里叶变换红外光谱、差示扫描量热法、卢里亚-贝尔塔尼培养基液体培养、人工模拟胃肠液体外消化法，测定包埋材料之间的相容性、包埋凝结芽孢杆菌后的热稳定性、在液体培养过程中的pH变化差异、在人工模拟胃肠液中体外消化特性（溶胀率、膨胀行为、pH值敏感性）。结果表明，SA与CN具有良好的相容性；纳米纤维素的添加有效地增强了SA-CN-BC结构的热稳定性；液体培养中经过包埋的凝结芽孢杆菌pH值变化更小，表明其显著起到缓释的作用，且当SA和CN复配比例为1:1时，凝胶球网络的疏松度最大，溶胀率最大，达到3243%；SA-CN-BCs在模拟胃液中皱缩，在模拟肠液中膨胀，具有显著的pH值敏感性。本研究成功制备了一款具有pH值敏感性的海藻胶-纳米纤维素水凝胶球，可用于递载益生菌的凝胶球，为水凝胶作为药物和营养传递生物相容性载体提供更多的可能性。

In order to improve the tolerance of probiotics delivery to strong acids, digestive enzymes and bile salts in the gastrointestinal tract, Cellulose Nanofibril (CN) and Sodium Alginate (SA) were compounded. By Fourier transform infrared spectroscopy, differential scanning calorimetry, Luria-Bertani medium liquid culture, artificial simulated gastrointestinal liquid digestion method, The compatibility between the embedding materials, the thermal stability after embedding Bacillus coagulum, the difference in pH change during liquid culture, and the in vitro digestion characteristics (swelling rate, swelling behavior, and pH sensitivity) in artificial simulated gastrointestinal fluids were measured. The results showed that SA had good compatibility with CN. The addition of nanocellulose effectively enhanced the thermal stability of SA-CN-BC structure. In the liquid culture, the pH value of the embedded Bacillus coagulans changed less, indicating that it played a significant role in sustained release. When the ratio of SA and CN was 1:1, the looseness of the gel sphere network was the largest, and the swelling rate was the largest, reaching 3243%. SA-CN-BCs shrank in simulated gastric fluid and distend in simulated intestinal fluid, showing significant pH sensitivity. This study successfully prepared a ph-sensitive alginate gel-nanocellulose hydrogel sphere, which can be used to deliver probiotics, providing more possibilities for hydrogels as biocompatible carriers for drug and nutrient delivery.

国家自然科学基金青年科学基金项目（31701630）；中国科学研究院过程工程研究所-百合股份联合培养博士后项目；辽宁省教育厅高等学校基本科研项目面上项目（LJKZ0715）；江苏省高校“青蓝工程”中青年学术带头人项目(2024)；江苏省高职院校教师专业带头人高端研修项目资助（2023GRFX061）；2022年青年教师科研能力提升计划（BS[2022]18）；苏州市乡村振兴软科学重点课题（SN-XCZX-2023-04）；校级科研项目（ZQZR202425）