该文采用超声耦合亚临界水法提取香菇多酚，考察香菇多酚对α-葡萄糖苷酶的抑制效果，并结合荧光光谱法分析香菇多酚体外降糖的抑制作用。结果表明，香菇多酚对α-葡萄糖苷酶的半抑制浓度IC50值为0.41 mg/mL，在0.1~1.5mg/mL的质量浓度范围内，香菇多酚对α-葡萄糖苷酶的抑制作用呈现剂量依赖性，并且在香菇多酚质量浓度达1.5mg/mL时，抑制活性达到79.22%，具有与阿卡波糖相近的降糖效果。荧光光谱结果显示，香菇多酚对α-葡萄糖苷酶的抑制类型为竞争型抑制，抑制活性随温度（298、303、310 K）的升高而降低，对α-葡萄糖苷酶内源性荧光具有静态猝灭作用，与α-葡萄糖苷酶的结合位点数为0.35。综上所述，香菇多酚能够与α-葡萄糖苷酶结合从而产生体外降糖活性，具有开发降糖的香菇类功能性产品的潜力。

In this paper, the inhibition effect of Lentinus edodes polyphenol on α-glucosidase is investigated by ultrasonic coupled subcritical water method, and the inhibitory effect of Lentinus edodes polyphenol hypoglycemia in vitro was analyzed by fluorescence spectrometry. The results showed that the semi-inhibitory concentration IC50 value of Lentinus edodes polyphenolon α-glucosidase was 0.41mg/mL, and the inhibitory effect of Lentinus edodes polyphenol on α-glucosidase was dose-dependent in the mass concentration range of 0.1-1.5 mg/mL. In addition, when the mass concentration of Lentinus edodes polyphenol reached 1.5mg/mL, the inhibitory activity reached 79.22%, so that the hypoglycemic effect was similar to acarbose. The results of fluorescence spectrum showed that the inhibition type of Lentinus edodes polyphenol was competitive inhibition, and the inhibitory activity decreased with the increase of temperature (298, 303, 310 K). The endogenous fluorescence of α-glucosidase had a static quenching effect, and the number of binding sites to α-glucosidase was 0.35. In summary, Lentinus edodes polyphenol can bind to α-glucosidase to produce in vitro hypoglycemic activity, which has the potential to develop functional Lentinus edodes products with hypoglycemic properties.