该研究旨在探讨雨生红球藻破壁孢子粉对紫外线B(UVB)引起光损伤的保护作用。通过UVB辐照诱导构建HaCaT细胞损伤模型,并以雨生红球藻破壁孢子粉进行干预,采用CCK8法检测细胞的增值,比色法检测细胞中的超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量；以UVB辐照引起斑马鱼损伤,测量添加雨生红球藻破壁孢子粉对尾鳍面积的影响,并以吖啶橙染色确定细胞凋亡情况。结果表明:雨生红球藻破壁孢子粉(12.5 μg/mL、25.0 μg/mL和50.0 μg/mL)干预将HaCaT细胞的存活率显著提高至84.47%、89.44%和96.30%,其中50.0 μg/mL的雨生红球藻破壁孢子粉处理使细胞中SOD活力比模型组提高了35.48%,MDA含量水平降低至模型组的23.05%；与模型组比较,0.125 mg/mL和0.1875 mg/mL雨生红球藻破壁孢子粉使UVB损伤斑马鱼的尾鳍面积显著增加了20.07%和 31.04 %,细胞凋亡率减少了 72.15 %和74.59%。雨生红球藻破壁孢子粉具有显著的光保护功效,可改善UVB诱导的氧化损伤,减少细胞凋亡,为其进一步的功能活性探索和开发应用提供了依据。

The study aims to investigate the protective effect of Haematococcus pluvialis spore powder on photodamage induced by ultraviolet B (UVB) radiation. A UVB-irradiated HaCaT cell damage model was established, and H. pluvialis broken spore powder was used for intervention. Cell proliferation was assessed using the CCK8 assay, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the cells were measured using colorimetric methods. UVB irradiation was used to induce damage in zebrafish, and the effect of adding H. pluvialis spore powder on tail fin area was measured, with acridine orange staining used to determine the extent of cell apoptosis. The results showed that intervention with H. pluvialis spore powder (12.5 μg/mL, 25.0 μg/mL, and 50.0 μg/mL) significantly increased the survival rate of HaCaT cells to 84.47%, 89.44%, and 96.30%, respectively. Treatment with 50.0 μg/mL H. pluvialis broken spore powder increased SOD activity in the cells by 35.48% compared to the model group, and reduced MDA content to 23.05% of the model group. Compared with the model group, 0.125 mg/mL and 0.1875 mg/mL H. pluvialis spore powder significantly increased the tail fin area of UVB-damaged zebrafish by 20.07% and 31.04%, respectively, and reduced the apoptosis rate by 72.15% and 74.59%. H. pluvialis spore powder has significant photoprotective effects, improves UVB-induced oxidative damage, and reduces cell apoptosis, providing a basis for further exploration of its functional activity and potential applications.

海南省自然科学(322RC590)；国家自然科学(32460929)；海南大学全健康协同创新中心项目(XTCX2022JKB08)