[关键词]
[摘要]
研究新疆特色植物资源疏花蔷薇花不同极性部位的体外抗氧化活性,为该药材的后续研究提供参考。制备疏花蔷薇花的石油醚(E1)、二氯甲烷(E2)、乙酸乙酯(E3)、正丁醇(E4)等不同极性部位提取物,考察其对DPPH、ABTS+和羟基自由基的清除能力评价体外抗氧化活性;以抗氧化活性最高的E4为研究对象,以D-半乳糖诱导小鼠巨噬细胞RAW264.7建立体外氧化损伤模型,设立对照组、模型组、Vc组、E4(低、中、高剂量)组,CCK-8法测定细胞存活率,测定细胞中超氧化物歧化酶、谷胱甘肽过氧化物酶、乳酸脱氢酶、过氧化氢酶的活力及丙二醛含量。疏花蔷薇花不同极性部位抗氧化活性大小为E4>E3>E2>E1,其中E3、E4清除自由基能力较强,并且呈明显剂量依赖效应。当E4质量浓度为3.13~12.50 μg/mL时,RAW264.7细胞的存活率均≥80%,无明显毒性;各剂量组可显著促进RAW264.7细胞存活率(P<0.05),并呈剂量依赖性提高细胞中SOD、GSH-PX、LDH、CAT活力,降低MDA含量。结果表明疏花蔷薇花具有良好的体外抗氧化能力。
[Key word]
[Abstract]
The in vitro antioxidant activities of different polar parts of the flowers of Rosa laxa Retz., a characteristic plant resource of Xinjiang, were studied to provide a reference for the subsequent research of this medicinal material. Extracts of different polar parts of the flower using petroleum ether (E1), dichloromethane (E2), ethyl acetate (E3) and n-butanol (E4) were prepared and their in vitro antioxidant activities were evaluated by investigating their scavenging abilities on DPPH, ABTS+, and hydroxyl radicals. E4 with the highest antioxidant activity among them was used as the research object for subsequent studies. An in vitro oxidative damage model of mouse macrophage RAW264.7 induced by D-galactose was established. The control group, model group, Vc group and E4 groups (low-, medium- and high-dose groups) were set up. The cell survival rate was determined by the CCK-8 method, and the activities of superoxide dismutase, glutathione peroxidase, lactate dehydrogenase and catalase in cells, and the content of malondialdehyde in cells, were determined. The antioxidant activities of different polar parts of the Rosa laxa Retz. flower were in the order of E4>E3>E2>E1, among which E3 and E4 exhibited strong free radical scavenging abilities and showed obvious dose-dependent effects. At an E4 mass concentration of 0.5~10 μg/mL, the survival rate of E4 on RAW264.7 cells was ≥ 80%, with no obvious toxicity detected. The treatments at each dose could significantly promote the survival rate of RAW264.7 cells (P<0.05), and in a dose-dependent manner, increase the activities of SOD, GSH-PX, LDH and CAT in cells while reducing the content of MDA. The above results demonstrated that the flowers of Rosa laxa Retz. possessed an in vitro good antioxidant activity.
[中图分类号]
[基金项目]
国家自然科学基金项目(81860744);新疆维吾尔自治区“十四五”重点学科:中医学