构树穗多糖的抗氧化活性及对AAPH诱导的人红细胞氧化损伤保护作用

Antioxidant Activity of Polysaccharide from Broussonetia papyrifera panicle and Its Protective Effect on Oxidative Damage of Human Red Blood Cells Induced by AAPH

摘要:

该文研究了构树穗多糖的抗氧化活性及对AAPH诱导的人红细胞氧化损伤保护作用。该研究以构树穗为原料,采用热水浸提法设计单因素试验,用响应面法优化得到最佳粗多糖提取工艺。以ABTS自由基、DPPH自由基、羟基自由基清除率,Fe3+还原能力和对AAPH诱导的人红细胞氧化损伤保护作用为考察指标,研究构树穗多糖的体外抗氧化活性和抑制人红细胞溶血性能。实验表明,最佳的提取工艺条件为：料液比1:32.33 g/mL,提取温度89.89oC,提取时间116.53 min,此时构树穗粗多糖得率23.67%；在抗氧化实验中,构树穗多糖对ABTS自由基、DPPH自由基、羟基自由基清除率分别达到了76.92%,57.81%,80.76%。构树穗多糖对Fe3+的还原能力在浓度8 mg/mL,波长700 nm条件下的吸光度为1.668；红细胞溶血实验表明,构树穗多糖可以有效地抑制AAPH处理的红细胞内ROS和MDA的产生,并降低GSH转化为GSSG的转化率,同时调节CAT、GSH-Px和SOD的酶活性恢复到正常红细胞的水平。研究证明了构树穗多糖具有良好的抗氧化能力,可以保护红细胞免受AAPH造成的氧化损伤。

Abstract:

In this paper, the antioxidant activity and protective effect of polysaccharides from Broussonetia papyrifera panicle on human erythrocyte oxidative damage induced by AAPH were studied. In this study, the single factor experiment was designed with hot water extraction method and the optimal extraction process of crude polysaccharide was optimized by response surface method. The antioxidant activity and hemolysis inhibition of Broussonetia papyrifera panicle polysaccharide in vitro were studied with the scavenging rate of ABTS free radical, DPPH free radical and hydroxyl free radical, the reducing ability of Fe3+ and the protective effect on human red blood cell oxidative damage induced by AAPH. The results showed that the optimal extraction conditions were as follows: solid-liquid ratio 1:32.33 g/mL, extraction temperature 89.89oC, extraction time 116.53 min, and the yield of crude polysaccharide was 23.67%. The scavenging rates of ABTS free radical, DPPH free radical and hydroxyl free radical were 76.92%, 57.81% and 80.76%, respectively. The absorbance of the polysaccharide to Fe3+ was 1.668 at the concentration of 8 mg/mL and the wavelength of 700 nm. Red blood cell hemolysis experiment showed that polysaccharides could effectively inhibit the production of ROS and MDA in red blood cells treated by AAPH, reduce the conversion rate of GSH to GSSG, and regulate the enzyme activities of CAT, GSH-PX and SOD to normal red blood cell levels. It has been proved that the polysaccharides of Broussonetia papyrifera panicle have good antioxidant capacity and can protect red blood cells from oxidative damage caused by AAPH.