该文基于牛乳酪蛋白水解物和灵芝多糖的免疫调节功效，探究其复配物对巨噬细胞和小鼠的免疫调节作用及其潜在协同效应。利用低中高剂量（100、200、500 μg/mL）复配物样品及其单组分（200 μg/mL）样品，测定巨噬细胞增殖能力、吞噬能力、分泌NO、IL-6、TNF-α的能力，测定小鼠免疫脏器指数、淋巴细胞增殖能力、迟发型变态反应程度和NK细胞活性。结果显示：与空白组相比，中高低剂量复配物、灵芝多糖和牛乳酪蛋白水解物均能显著提高巨噬细胞的吞噬能力（116.78%、133.47%、137.73%、131.07%、124.63%）、NO释放量（22.19、27.77、29.49、28.82、26.17 μm）、IL-6释放量（0.317、0.323、0.293、0.309、0.297 μg/mL）、TNF-α释放量（0.42、0.50、0.54、0.52、0.47 μg/mL）（P<0.05）。复配物低中高剂量均能增加小鼠足趾厚度差（0.305、0.354、0.429 mm）、提高脾淋巴细胞转化能力（25.30%、34.20%、36.13%）、NK 细胞活性（0.36、0.53、0.74）（P<0.05）。研究结果表明，复配物能促进巨噬细胞和小鼠的免疫调节作用，并且存在一定的协同作用。

The immunomodulatory efficacy of bovine casein hydrolysate and Ganoderma lucidum polysaccharides were studied, focusing on the immunomodulatory effects of their complex on macrophages and mice, along with their potential synergistic effects. Using low, medium, and high doses (100, 200, and 500 μg/mL) of the complex and its singlecomponent (200 μg/mL), the proliferation capacity, phagocytosis, secretion of NO, interleukin (IL)-6, and tumor necrosis factor alpha (TNF-α) of macrophages were determined. Additionally, the immune organ index, proliferation capacity of lymphocytes, degree of delayed-type hypersensitivity, and natural killer (NK) cell activity were measured in mice. The results showed that, compared to the blank group, low, medium and high dose complexes, G. lucidum polysaccharides, and bovine casein hydrolysates all significantly increased the phagocytosis capacity of macrophages (116.78%, 133.47%, 137.73%, 131.07%, and 124.63%, respectively), with NO release (22.19, 27.77, 29.49, 28.82, and 26.17 μm, respectively), IL-6 release (0.317, 0.323, 0.293, 0.309, and 0.297 μg/mL, respectively), and TNF-a release (0.42, 0.50, 0.54, 0.52, and 0.47 μg/mL, respectively) (P<0.05). The complexes with low, medium, and high doses all increased the difference in toe thickness (0.305, 0.354, and 0.429 mm, respectively), enhanced spleen lymphocyte's transformation capacity (25.30%, 34.20%, and 36.13%, respectively), and enhanced NK cell activity (0.36, 0.53, and 0.74, respectively) in mice (P<0.05). The study results indicated that the complexes promoted immunomodulatory effects in macrophages and mice and exhibited a synergistic effect.

湖南省创新平台与人才计划（2019TP2066）