[关键词]
[摘要]
为比较绿茶与茉莉花茶代谢差异,分别选取三种不同地域绿茶和茉莉花茶样品,利用超高效液相色谱-高分辨质谱联用技术对茶叶代谢物进行非靶向分析。共检测到177种代谢物,对其进行非参数检验Kruskal-Wallis检验,共筛选出59种代谢物,通过皮尔逊聚类分析归为3组。富集分析发现代谢差异集中分布在多酚、黄酮和氨基酸相关通路中。偏最小二乘判别分析结果证明不同产地绿茶和茉莉花茶可在得分散点图上被良好区分,模型参数变量投影重要性预测值筛选出差异代谢物28种。样品总多酚黄酮含量与差异代谢物相关分析发现呈最强正相关的是γ-氨基丁酸,最强负相关的是单酰基甘油磷酰胆碱18:0。对不同产地绿茶和茉莉花茶进行分析分别发现有10和55种差异代谢物。12-氧代植物二烯酸、茉莉酮和间苯三酚在安徽、江西和陕西绿茶中含量最高,苯乙酮、单二酰基甘油三甲基高丝氨酸16:0和2-己烯醛在安徽、广西和四川茉莉花茶中含量最高。本研究为茶叶滋味形成及不同产地茶叶鉴定提供了一定参考价值。
[Key word]
[Abstract]
To explore the metabolic differences between green and jasmine tea, samples of green tea and jasmine tea from three different regions were selected respectively, as well as performed the ultra-high-performance liquid chromatography-high resolution mass spectrometry based untargeted metabolomics analysis. The results indicated that 177 metabolites were detected totally and Kruskal-Wallis tests were performed, 59 metabolites were collected out. Then, Pearson cluster analysis was performed on changed metabolites and heatmap shown 3 groups were distinguished with different trends. Besides, metabolic pathway enrichment analysis results indicated that changed pathways mainly focused on polyphenol, flavone, and amino acid metabolism. To further reduce the scope of crucial metabolites, partial least squares-discriminant analysis was conducted, score scatter plot shown jasmine tea could be clearly separated from green tea. After filtering by the variable importance in the projection values, 28 differential metabolites were retained. In the correlation analysis of the total polyphenol and flavonoid content and differential metabolites in the sample, it was found that the strongest positive correlation was γ-aminobutyric acid, and the strongest negative correlation was lyso-phosphatidylcholine 18:0. Finally, the differences of green tea and jasmine tea from different producing places were analyzed and 10 and 55 differential metabolites were found, respectively. Among them, 12 oxo-phytodienoic acid, jasmonic acid, and phloroglucinol have the highest content in Anhui, Jiangxi, and Shaanxi green tea. Acetophenone, lyso-diacylglyceryltrimethylhomoserine 16:0, and 2-hexenal have the highest content in Anhui, Guangxi, and Sichuan jasmine tea. This study provide reference for the research on the formation of tea taste and identification of tea from different producing areas.
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[基金项目]