建立了高效液相色谱-四级杆/静电场轨道肼高分辨率质谱法测定禽蛋中阿苯达唑及其代谢物的检测方法。禽蛋样品经乙腈溶液提取、无水硫酸钠除水、正己烷脱脂净化、氮吹后，用体积比为3:7的甲醇水溶液定容并溶解残渣，充分涡旋混合后过0.45 μm滤膜。采用Phenomenex C18（100 mm×4.6 mm, 2.6 μm）色谱柱进行分离，以乙腈-0.1%甲酸水溶液（体积分数）为流动相梯度洗脱。质谱采用正离子模式，扫描范围：100~1 000 m/z，二级采用Target-ms/ms扫描模式，在碰撞能为30 eV和分离窗口m/z为2.0的条件下，提取响应值较高且在待测样品中无干扰的目标化合物的精确质量数，以二级子离子为定量离子，外标法定量。在所建立的色谱条件下，阿苯达唑及三种代谢物能够得到较好的分离。该方法对阿苯达唑及代谢物的检测限可达3 μg/kg，定量限可达10 μg/kg，在20~200 ng/mL浓度范围内线性关系良好，相关系数均大于0.99。通过加标验证，在三个水平下的平均回收率为75.9%~97.4%，相对标准偏差（Relative Standard Deviations, RSD）为4.3%~8.5%。该方法样品处理过程简单，回收率高、稳定性好，可以用于禽蛋中阿苯达唑及其代谢的测定，为规范禽蛋中阿苯达唑兽药的使用具有重要意义。

A method based on high performance liquid chromatography-quadrurpole/electrostatic field orbitrap high-resolution mass spectrometry (HPLC-Q/Qrbitrap MS) was developed for the determination of albendazole and its metabolites in poultry eggs. The egg samples were extracted with acetonitrile. The obtained extracts were dehydrated by anhydrous sodium sulfate, degreased by n-hexane and purified by nitrogen blowing. The residue was dissolved in methanol-water solution (volume ration: 3:7, V/V), followed by well vortexing then filtration through 0.45-μm membrane filters. A Phenomenex C18 column (100 mm×4.6 mm, 2.6 μm) was used to separate the analytes by gradient elution using acetonitrile-0.1% (volume fraction) formic acid aqueous solution as the mobile phase. MS scanning experiments were performed over the range of m/z 100~1 000 in positive mode. The Target-MS/MS scanning mode was used for the second stage. The collision energy was 30 eV and the separation window m/z was 2.0. The mass number of the target compound was accurate with high response value, and no interference in the to-be-analyzed sample was detected by the external standard method with the secondary ion as quantitative ion. The samples were determined based on external standard method with secondary quantitative ion. Under the established chromatographic conditions, albendazole and the three metabolites were well separated. The detection limits and quantification limits were 3 μg/kg and 10 μg/kg, respectively. Good linear relationship was obtained in the range of 20~200 ng/mL, and the correlation coefficients were all higher than 0.99. Spiking experiments were performed for verification, and the average recoveries at three levels were in the range of 75.9%~97.4% with the relative standard deviations (RSD) in the range of 4.3%~8.5%. The proposed method was simple, with a high recovery rate and good stability, thus, can be used for the determination of albendazole and its metabolites in poultry eggs. The method is of great significance for regulating albendazole veterinary drugs in poultry eggs.

海关总署课题（2021HK192）；上海市“科技创新行动计划”技术标准项目（21DZ2208500）