该文以骏枣、灰枣、药枣和若羌枣为研究对象，水提醇沉法制备得JP、HP、YP、RP四种红枣多糖，并对其进行结构和功能的对比研究。结果表明多糖的得率为5.71%~7.01%，红枣多糖均为酸性多糖，其糖醛酸含量为34.80%~48.61%；分子量为：29.17 kDa~140.71 kDa；由不同摩尔比的半乳糖、葡萄糖、阿拉伯糖、鼠李糖、木糖及甘露糖构成主要以半乳糖及阿拉伯糖为主；紫外和红外吸收光谱表现出具有多糖的特征吸收峰。红枣多糖具有较好的持水、持油、乳化性及生物活性。YP对于清除1-1-二苯基-2-三硝基苯肼、2-2,-联氮-双（3-乙基苯并噻唑啉-6-磺酸）和羟自由基的清除能力较强，半抑制浓度分别为0.20、7.00×10-2、0.59 (mg/mL)。红枣多糖对巨噬细胞RAW264.7显出不同的细胞增殖活性及分泌细胞因子的能力，其中JP具有明显的促进细胞增殖作用（P<0.05），均显著促进细胞分泌IL-1β（P＜0.01），RP和HP显著促进细胞分泌TNF-α（P＜0.01）。综上，不同的红枣多糖应用潜力各异，该研究将为不同品种红枣多糖构效关系的研究及红枣资源的高值化开发利用提供参考。

In this research, four different kinds of Ziziphus jujuba including Junzao, Huizao, Yaozao, and Ruoqiangzao as research objects, and four types of jujube polysaccharides JP, HP, YP, and RP were prepared by water extraction and alcohol precipitation method, then their structures and functional properties were compared. The yield of polysaccharides was ranging in 5.71%~7.01%. All the polysaccharides were acidic polysaccharides with the uronic acid content of 34.80%~48.61%. Molecular weight was 29.17 kDa~140.71 kDa; Ziziphus jujuba polysaccharides consisted of galactose, glucose, arabinose, rhamnose, xylose, and mannose with a different molar ratio. Ziziphus jujuba polysaccharides have better water holding, oil holding, emulsifying properties, and biological activity, YP has stronger free radical scavenging abilities against 1-1-diphenyl-2-trinitrophenylhydrazine, 2-2,-diazo-bis (3-ethylbenzothia zolin-6-sulfonic acid), and hydroxyl radicals with IC50 values of 0.20, 7.00×10-2, and 0.59 (mg/mL), respectively. Ziziphus jujuba polysaccharides showed different cell proliferation activities and cytokine secretion abilities on macrophages RAW264.7. Among them, JP had significant promoting effects on cell proliferation (P<0.05), all polysaccharides significantly promote the secretion of IL-1β (P<0.01), and RP and HP have significant effects on promoting the secretion of TNF-α (P<0.01). In conclusion, the results showed that Ziziphus jujuba polysaccharides have different application potential. This study will provide a reference for the study on the structure-activity relationship of Ziziphus jujuba polysaccharides and the further development and utilization of this resources.

新疆维吾尔自治区“三区人才”项目