[关键词]
[摘要]
为有效利用福白菊的非药用部位,实现菊花资源综合开发利用,以不同采收期不同部位的福白菊为实验材料,系统测定并评价了其有效成分含量。同时利用1,1-二苯基-2-三硝基苯肼(DPPH)自由基和2'-联氮双(3-乙基苯和噻唑林-6-磺酸)二胺盐(ABTS)自由基清除力和铁离子还原力(FRAP)3种抗氧化指标分析评价其体外抗氧化能力。结果显示,福白菊不同部位有效成分的含量分别为粗脂肪9.13~52.18 mg/g,蛋白质62.72~189.50 mg/g,粗纤维138.26~459.12 mg/g,总氨基酸和必需氨基酸含量分别为8.19~45.32 mg/g,0.45~4.6 mg/g,总黄酮11.86~80.99 mg/g,总酚13.11~99.33 mg/g,中性多糖和酸性多糖含量分别为20.24~96.78 mg/g和9.46~43.28 mg/g。进一步氨基酸组成分析表明福白菊中味觉氨基酸占比较大为41.78%~56.19%,其次药用氨基酸为12.50%~46.72%;9个多酚类成分单体化合物分析,酚酸类化合物较黄酮类化合物含量高且差异性显著。通过主成分和模糊数学隶属函数法对有效成分综合分析,结果为苗(0.814)、叶(0.522)、花(0.378)、茎(0.058)。福白菊抗氧化综合结果为苗(65.76%)、叶(64.56%)、花(62.53%)、茎(61.91%)。综上实验结果在对福白菊非药用部位的应用上提供了有力的科技支撑,有助于进一步拓宽其应用领域。
[Key word]
[Abstract]
In order to effectively utilize the non medicinal parts of Chrysanthemum morifolium cv. Fubaiju and achieve comprehensive development and utilization of chrysanthemum resources, the content of its active ingredients was systematically measured and evaluated using C. morifolium cv. Fubaiju from different harvesting periods and parts as experimental materials. Simultaneously, the in vitro antioxidant capacity of the compound was evaluated using three antioxidant indicators: 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radical, 2 '- bis (3-ethylbenzene and thiazolin-6-sulfonic acid) diamine salt (ABTS) radical scavenging ability, and iron ion reducing ability (FRAP). The results showed that the content of active ingredients in different parts of Chrysanthemum morifolium was 9.13-52.18 mg/g crude fat, 62.72-189.50 mg/g protein, 138.26-459.12 mg/g crude fiber, 8.19-45.32 mg/g total amino acids, and 0.45-4.6 mg/g essential amino acids, respectively,11.86-80.99 mg/g total flavonoids, 13.11-99.33 mg/g total phenols, 20.24~96.78 mg/g neutral polysaccharides and 9.46~43.28 mg/g acidic polysaccharides, respectively. Further amino acid composition analysis showed that the proportion of taste amino acids in Chrysanthemum morifolium was 41.78%~56.19%, followed by medicinal amino acids at 12.50%~46.72%; Analysis of 9 polyphenolic monomers showed that phenolic acid compounds had higher content and significant differences compared to flavonoid compounds. The comprehensive analysis of effective components using principal component analysis and fuzzy mathematical membership function method resulted in seedlings (0.814), leaves (0.522), flowers (0.378), and stems (0.058). The comprehensive results of antioxidant antioxidant of Chrysanthemum were seedlings (65.76%), leaves (64.56%), flowers (62.53%) and stems (61.91%). In summary, the experimental results provide strong scientific and technological support for the application of non-medicinal parts of chrysanthemum, which is helpful to further broaden its application field.
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[基金项目]
国家重点研发计划(基于拟境栽培的中药材生态种植关键技术优化及品质提升;2023YFC3503804));湖北省重点研发计划(麻城福白菊良种高效繁育和规范化产地加工关键技术研究;2022BBA0020);中央本级重大增减支项目(No.2060302)