为提高川明参粗多糖（Chuanminshen violaceum Polysaccharides，CVPs）的生物活性，通过超声辅助酶法提取川明参粗多糖，采用浓硫酸法制备硫酸化川明参粗多糖（Sulfated Chuanminshen violaceum Polysaccharides，S-CVPs），利用DEAE-52及Sephadex G-100对硫酸化川明参粗多糖进行纯化，通过傅里叶红外光谱观察到纯化后的硫酸化多糖在1204 cm-1、816 cm-1处有硫酸基团的特征吸收，并且在扫描电子显微镜下观察到硫酸化多糖出现了条纹状缝隙，这样的结构更容易与水分子结合。HPGPC和HPLC分析结果显示，纯化的硫酸化川明参多糖组分（Purified Sulfated Chuanminshen violaceum Polysaccharide Components，S-CVPs-1-G）是一种分子量为4.2 kDa多糖聚合物，其组成成分为葡萄糖（Glc）占81.00%，半乳糖（Gal）占5.31%，甘露糖（Man）占4.54%，以及半乳糖醛酸（GalA）占3.52%。采用DPPH、ABTS及OH等自由基清除实验，考察了硫酸化多糖的体外抗氧化活性，结果显示，各组分的抗氧化能力由高到低为 S-CVP-1-G＞S-CVPs＞CVPs。综上所述，对CVPs进行硫酸化修饰可显著提升其体外抗氧化活性。此外，通过分离纯化获得的高纯度多糖物质亦能有效增强其抗氧化能力。研究结果可为川明参的进一步开发利用提供依据。

To enhance the biological activity of Chuanminshen violaceum polysaccharides (CVPs), these polysaccharides were extracted using an ultrasonic-assisted enzymatic method. The extracted CVPs were then sulfated using the concentrated sulfuric acid method to produce sulfated Chuanminshen violaceum polysaccharides (S-CVPs). The S-CVPs were purified using DEAE-52 and Sephadex G-100 chromatography. Fourier-transform infrared spectroscopy revealed characteristic absorption bands of sulfate groups at 1204 cm-1 and 816 cm-1 in the purified sulfated polysaccharides. Scanning electron microscopy showed that the sulfated polysaccharides exhibited striated gaps in their structure, which could more readily bind with water molecules. HPGPC and HPLC analysis showed that the purified sulfated Chuanminshen violaceum polysaccharide component (S-CVPs-1-G) is a polysaccharide polymer with a molecular weight of 4.2 kDa, composed of 81.00% glucose (Glc), 5.31% galactose (Gal), 4.54% mannose (Man), and 3.52% galacturonic acid (GalA). The in vitro antioxidant activity of the sulfated polysaccharides was assessed using DPPH, ABTS, and OH radical scavenging assays. The results showed that the antioxidant capacity of the components ranked as follows:S-CVP-1-G＞S-CVPs＞CVPs. In summary, sulfation of CVPs significantly enhances their in vitro antioxidant activity. Furthermore, the high purity polysaccharides obtained through isolation and purification also effectively enhance their antioxidant capacity. These findings provide a basis for further development and utilization of Chuanminshen violaceum.

重庆市自然科学基金面上项目;重庆市教委科研项目