以玫瑰茄为原料制备花青素，利用AmberliteXAD-7大孔树脂进行纯化、采用傅里叶红外光谱及超高效液相色谱串联飞行时间质谱对玫瑰茄花青素纯化物（Purified Roselle Anthocyanins，PRA）中花色苷进行鉴定，并评价PRA在体外和HepG2细胞内的抗氧化活性。结果表明，玫瑰茄花青素纯化后花青素含量提高了6.35倍，达到175.27 mg/g。PRA中含有五种花色苷，以飞燕草素-3-桑布双糖苷相对丰度最高，占总花色苷丰度的57.35%。PRA具有良好的体外抗氧化作用。当PRA质量浓度为0.23 mg/mL时，对1,1-二苯基-2-苦基肼自由基（1,1-diphenyl-2-picryl-hydrazil，DPPH）自由基清除率达90%；PRA质量浓度为2.0 mg/mL时，对羟自由基清除率达97.85%。PRA还具有良好的HepG2细胞内抗氧化活性。200 μg/mL PRA处理使H2O2诱导的HepG2细胞内活性氧水平从117.47%（以对照组2',7'-二氯荧光素荧光强度为100%）降低至102.09%，一氧化氮含量由53.18 nmol/mL降低至45.79 nmol/mL，超氧化物歧化酶活力由18.19 U/mg prot升高至35.05 U/mg prot，过氧化氢酶活力由10.10 U/mg prot升高至19.38 U/mg prot。综上，PRA花色苷以飞燕草素-3-桑布双糖苷为主，且具有良好的体外抗氧化活性和体内抗氧化应激能力。该研究结果为玫瑰茄花青素的高值化开发以及潜在健康功效提供理论依据。

Roselle was used as a raw material for extracting anthocyanins. The anthocyanins were purified using Amberlite XAD-7 macroporous resin. Purified roselle anthocyanins (PRA) were identified using Fourier-transform infrared spectroscopy and ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The antioxidant activity of PRA in vitro and in HepG2 cells was evaluated. The results showed that the anthocyanin content in PRA increased by 6.35-fold, reaching 175.27 mg/g. PRA contained five anthocyanins, of which delphinidin-3-sambubioside was the most abundant, accounting for 57.35% (m/m) of the total anthocyanins. PRA exhibited high antioxidant activity in vitro. The DPPH scavenging rate reached 90% when the PRA concentration was 0.23 mg/mL, and the hydroxyl radical scavenging rate was 97.85% at a PAR concentration of 2.0 mg/mL. Additionally, PRA showed high intracellular antioxidant activity in HepG2 cells. Treatment with 200 μg/mL PRA significantly reduced the H2O2-induced HepG2 intracellular reactive oxygen species level and nitric oxide content from 117.47% (taking the 2′,7′-dichlorofluorescein fluorescence intensity of the control group as 100%) and 53.18 nmol/mL to 102.09% and 45.79 nmol/mL, respectively. PRA treatment significantly increased the superoxide dismutase and catalase activities from 18.19 and 10.10 U/mg prot to 35.05 and 19.38 U/mg prot, respectively. The main component of PRA anthocyanins was delphinidin-3-sambubioside, which exhibited strong antioxidant activity in vitro and anti-oxidative stress response effects in vivo. These results provide a theoretical basis for the high-value development and the potential health benefits of roselle anthocyanins.

福建省中青年教师教育科研项目（JAT220065）；福建省高校产学合作项目（2021N5011）