研究益生菌发酵番茄汁（Probiotic Fermented Tomato Juice，PFTJ）的解酒护肝作用并揭示其作用机制。利用小鼠急性酒精中毒模型分析了不同剂量PFTJ 对小鼠醒酒时间和乙醇脱氢酶（Alcohol Dehydrogenase，ADH）的影响，利用慢性酒精中毒模型检测了其对小鼠血清中肝功能指标、脂质代谢指标和肝组织中抗氧化指标的影响，并进行了组织病理学分析。结果表明，PFTJ能够有效缩短急性酒精中毒小鼠醒酒时间，提高ADH活性；与模型组相比，高剂量组（15 mL/kg）小鼠谷草转氨酶（Aspartate Transaminase，AST）、谷丙转氨酶（Alanine Aminotransferase，ALT）活性分别降低了24.87%和26.65%（P<0.05），总胆固醇（Triglyceride，TG）、甘油三酯（Total Cholesterol，TC）则分别降低至0.58 mmol/L和1.21 mmol/L，肝组织中过氧化氢酶（Catalase，CAT）、超氧化物歧化酶（Superoxide Dismutase，SOD）活性分别提高了17.05%和22.91%（P<0.05），丙二醛（Malondialdehyde，MDA）含量降低了34.69%（P<0.05）。灌胃15 mL/kg PFTJ能够显著抑制酒精损伤小鼠肝细胞肿大和脂肪变性。因此，PFTJ可提高ADH活性增加乙醇代谢速率，提高抗氧化酶活性清除肝脏自由基，恢复脂质代谢水平，有潜力成为一种新的解酒护肝功能性食品。

The efficacy and mechanisms of probiotic fermented tomato juice (PFTJ) in mitigating the impact of alcohol, and its ability to protect the liver were examined. The effects of different doses of PFTJ on the time taken by mice to sober up and on the activity of alcohol dehydrogenase (ADH) in mice were analyzed using mouse models of acute alcohol intoxication. In the meantime, models of chronic alcohol intoxication were established to investigate the effects of PFTJ on the liver function indicators and lipid metabolism indicators in serum as well as the antioxidant indicators in liver tissue of mice. In addition, histopathological analysis was conducted. The results showed that PFTJ significantly shortened the time taken by mice to sober up and improved ADH activity in acute alcohol intoxication models. Compared with those of the model group, the activities of aspartate transaminase (AST) and alanine aminotransferase (ALT) decreaseds by 24.87% and 26.65%, respectively (P<0.05), and the levels of triglyceride (TG) and total cholesterol (TC) reduced to 0.58 mmol/L and 1.21 mmol/L, respectively, in the high-dose group (15 mL/kg). The activities of catalase (CAT) and superoxide dismutase (SOD) in liver tissue increased by 17.05% and 22.91%, respectively (P<0.05), while the content of malondialdehyde (MDA) declined by 34.69% (P<0.05). The administration of 15 mL/kg PFTJ remarkably relieved liver cell swelling and steatosis in the mouse models. In conclusion, PFTJ can increase ADH activity to accelerate ethanol metabolism and enhance activities of antioxidant enzymes to eliminate free radicals in the liver and restore lipid metabolism. Therefore, PFTJ has the potential to become a new functional food for sobering up and liver protection.

新疆生产建设兵团中央引导地方科技发展资金项目（2023）；河北省重点研发计划项目（22322801D）