Abstract:To explore the impact of inulin on the redox status and intestinal flora in mouse models of eczema, we utilized thirty male Kunming mice. These mice were randomly assigned to the control, model, and high- and low-dose inulin treatment groups, with interventions of varying insulin doses administered accordingly. Except for the control group, each group was sensitized with 2,4-dinitrochlorobenzene (DNCB) to establish eczema models. Inulin was added into the feed at mass fractions of 5% and 20% for high-and low-dose groups, respectively, for four weeks. HE staining was used to observe pathological changes in the skin and colon. The activity of total superoxide dismutase (T-SOD) and levels of malondialdehyde (MDA) in spleen and thymus were determined using biochemical methods. Feces were collected for 16S rRNA highthroughput sequencing to analyze the composition of gut microbiota in mice. The pathological results indicated that the changes in the skin and colon in mice were alleviated after inulin intervention. Comparison of the treatment groups with the model group revealed that inulin at a high dose significantly increased T-SOD and reduced MDA content in spleen and thymus (P<0.05). In addition, the abundance of Bifidobacterium, Allobaculum, and Olsenella in the low-dose group increased considerably (P<0.05), and the abundance of Allobaculum in the high-dose group increased significantly (P<0.05). Inulin at both low and high doses significantly reduced the abundance of Enterococcus. In short, inulin can effectively improve eczema by regulating gut microbiota and improving antioxidant capacity.
