研究了菊粉对湿疹的改善作用，并探究其对湿疹小鼠氧化还原状态和肠道菌群的影响。30只雄性昆明小鼠随机分为对照组、模型组、菊粉低剂量和高剂量干预组，除对照组外，各组采用2,4-二硝基氯苯（DNCB）致敏建立湿疹模型。将菊粉按质量分数5%和质量分数20%添加到饲料中，连续干预4周。HE染色观察皮肤和结肠组织病理变化；生化检测脾脏和胸腺组织中总超氧化物歧化酶（T-SOD）活性及丙二醛（MDA）水平；收集粪便采用16S rRNA测序技术进行肠道菌群分析。结果表明，菊粉干预后小鼠皮肤和结肠病理改变得到改善；与模型组相比，高剂量菊粉能显著提高胸腺和脾脏中T-SOD酶活性，同时降低MDA含量（P<0.05）。此外，与模型组比较，菊粉低剂量组双歧杆菌属（Bifidobacterium）、Allobaculum和Olsenella丰度显著增加（P<0.05），菊粉高剂量组Allobaculum丰度显著增加（P<0.05），菊粉高、低剂量组的肠球菌（Enterococcus）的丰度均显著降低（P<0.05）。该结果说明菊粉能有效改善湿疹，调节肠道菌群紊乱并提高机体抗氧化能力。

To explore the impact of inulin on the redox status and intestinal flora in mouse models of eczema, we utilized thirty male Kunming mice. These mice were randomly assigned to the control, model, and high- and low-dose inulin treatment groups, with interventions of varying insulin doses administered accordingly. Except for the control group, each group was sensitized with 2,4-dinitrochlorobenzene (DNCB) to establish eczema models. Inulin was added into the feed at mass fractions of 5% and 20% for high-and low-dose groups, respectively, for four weeks. HE staining was used to observe pathological changes in the skin and colon. The activity of total superoxide dismutase (T-SOD) and levels of malondialdehyde (MDA) in spleen and thymus were determined using biochemical methods. Feces were collected for 16S rRNA highthroughput sequencing to analyze the composition of gut microbiota in mice. The pathological results indicated that the changes in the skin and colon in mice were alleviated after inulin intervention. Comparison of the treatment groups with the model group revealed that inulin at a high dose significantly increased T-SOD and reduced MDA content in spleen and thymus (P<0.05). In addition, the abundance of Bifidobacterium, Allobaculum, and Olsenella in the low-dose group increased considerably (P<0.05), and the abundance of Allobaculum in the high-dose group increased significantly (P<0.05). Inulin at both low and high doses significantly reduced the abundance of Enterococcus. In short, inulin can effectively improve eczema by regulating gut microbiota and improving antioxidant capacity.

国家大学生创新创业训练计划项目（202010601033）；广西自然科学基金资助项目（2021GXNSFBA075038）；广西高等学校千名中青年骨干教师培育计划（桂教教师[2020]58号）