Abstract:The nutritional components of flowers (capitulums) of Hangzhou white chrysanthemum and its F1 hybrid superior lines were analyzed using liquid chromatography-mass spectrometry (LC-MS) technology. The results showed that 502, 505, and 496 metabolites were detected in Hangzhou white chrysanthemum, Hang F1-17, and Hang F1-22, respectively. A total of 309 common metabolites were found in the three chrysanthemums. Compared to Hangzhou white chrysanthemum, according to the fold change, namely FC≥1.5 and ≤0.8, which indicated significant up-regulation and down-regulation, there were 135 and 206 significantly up-regulated metabolites detected in Hangzhou F1-17 and Hangzhou F1-22 respectively, and there were 144 and 154 significantly down-regulated metabolites, respectively. Among the significantly changed metabolites of Hang F1-17 and Hang F1-22, the relative contents of three flavonoid metabolites of kaempferol (FC of 16.77 and 18.69), luteolin (FC of 7.35 and 3.74), isorhamnetin (FC of 3.21 and 6.38); methionine (FC of 103.22 and 67.71), one of the essential amino acids for the human body; the main organic acids of malic acid (FC of 306.73 and 2.76) and citric acid (FC of 4.82 and 12.16); and chlorogenic acid (FC of 1.53 and 3.11), the main health component in chrysanthemum, have all been significantly up-regulated. Additionally, when compared to the female parent Hangzhou white chrysanthemum, components such as ursolic acid, vitamin A, and K3 were only detected in Hang F1-17, while components such as carnosol, myricetin, and vitamin B1 were only detected in Hang F1-22. These results indicate that relative contents of some main nutritional components in the F1 hybrids are obviously higher than that in the female parent, and some specific nutritional components are only detected in the F1 hybrids. Meanwhile, the anemone-typed capitulum of Hang F1-17 also provide references for the development of excellent tea chrysanthemum cultivars for both ornamental and food uses in the future.
