6''-唾液酸乳糖改善秀丽隐杆线虫运动能力和促进线虫神经发育

6''-Sialyllactose Improves the Locomotion Ability of Caenorhabditis elegans and Promotes the Neural Development

摘要:

神经发育不良导致的发育迟缓、行为障碍和认知障碍给婴幼儿和亲人带来巨大的痛苦和灾难,亟需开发一些能够改善婴幼儿运动协调和神经发育的活性物。6"-唾液酸乳糖(6"-Sialyllactose,6"-SL)是一种母乳低聚糖(Human Milk Oligosaccharides,HMOs),具有改善肠道健康、抗黏附、抗病毒和促进大脑发育等功效。本研究利用神经生物学理想模式生物-秀丽隐杆线虫(Caenorhabditis elegans,C.elegans)探究了6"-SL对神经发育促进功效和运动能力的改善功效。主要结果如下:在6"-SL在0.02-1.0 mg/mL范围内能够有效地延长线虫的瘫痪寿命。根据6"-SL在产品中的实际应用,选择0.56 mg/ml 6"-SL继续进行探究。0.56 mg/mL 6"-SL能够显著改善CL4176线虫的头摆频率、吞咽频率和N2线虫的运动速率。6"-SL在第7和11天能够显著保护线虫胆碱能神经元形态；此外,6"-SL使线虫乙酰胆碱酯酶活性下降了22.60%±8.0%,同时提高了线虫体内多种神经递质含量水平:多巴胺(dopamine,DA)水平提升了148.71%、5-羟色胺(serotonin,5-HT)水平提升了72.17 %、γ-氨基丁酸(4-aminobutyric acid,GABA)水平提升了34.72 %、谷氨酸(Glutamate,Glu)水平提升了68.47 %。综上所述,6"-SL可有效地改善运动能力和神经发育,为6"-SL在婴幼儿配方食品和保健品领域的应用提供理论支持。

Abstract:

The developmental delay, behavioral disorders and cognitive impairments caused by neurodevelopmental disorders bring enormous pain and disaster to infants and their families. Some active substances that can improve locomotion and neurodevelopment in infants and young children urgently need to be developed. 6"-Sialyllactose (6"-SL) is a human milk oligosaccharide (HMOs).It has the effects of improving intestinal health, anti adhesion, antiviral, and promoting brain development. This study investigated the effect of 6"-sialyllactose on promoting neural development and improving locomotion ability in Caenorhabditis elegans (C.elegans).The main results are as follows: within the range of 0.02-1.0 mg/mL, 0.56mg/ml 6"-SL can effectively prolong the paralysis life of nematodes;We have chosen 0.56 mg/ml 6"-SL to the follow researches based on the practical application of 6"-SL. 6"-SL can significantly improve the head swing frequency,pharyngeal pumping rate of CL4176, and improved average speed of N2. 6"-SL can significantly protect the morphology of cholinergic neurons in C.elegans on day 7 and day 11; In addition,6"-SL reduced the acetylcholinesterase activity by 22.60%±8.0%, while increasing the levels of various neurotransmitters in the nematode body: dopamine (DA) level increased by 148.71%, serotonin (5-HT) level increased by 72.17%, 4-aminobutyric acid (GABA) levels increased by 34.72%, while glutamate (Glu) levels increased by 68.47%. In summary, 6"-SL can effectively improve locomotion ability and neural development, providing theoretical support for the application of 6"-SL in infant formula and health products.