灰树花醇提物对秀丽隐杆线虫的抗氧化及降脂作用

Anti-oxidative and Lipid-lowering Effects of Alcohol Extracts from Grifola Frondosa in Caenorhabditis Elegans

摘要:

为研究灰树花醇提物的抗氧化及降脂作用，该研究首先测定了灰树花醇提物的总糖、蛋白、多酚和三萜含量，接着利用体外抗氧化实验和秀丽隐杆线虫模型分析其抗氧化能力。此外，通过油红O染色观察线虫体内脂质沉积情况，同时测定TG浓度和LPS活力探究其降脂潜力。结果发现灰树花醇提物主要以蛋白质为主，含量为233.67 mg/g；其对DPPH、ABTS和羟基自由基均有极显著清除效果，IC50值分别为3.25 mg/mL、0.38 mg/mL和1.73 mg/mL；与空白组相比，经灰树花醇提物干预的高剂量组的线虫体内抗氧化酶CAT、T-SOD和GSH-Px活力以及LPS活力分别极显著提升了4.33、4.88、2.28和3.93倍；线虫脂肪沉积水平、MDA和TG含量显著下降，高剂量组的MDA、TG水平分别下降了55.62%和50.66%。综上，灰树花醇提物具有体外抗氧化能力，对秀丽隐杆线虫体内抗氧化酶活力具有提升作用；降低线虫体内的脂质沉积水平和TG浓度，表明其具有良好的降脂效果。该研究可为灰树花高值化利用提供理论依据。

Abstract:

The antioxidant and lipid-lowering effects of the alcohol extract of Grifola frondosa (G. frondosa) were carried out. The composition content of the alcohol extract was determined first, then the antioxidant capacity was analyzed through the vitro antioxidant experiment and Caenorhabditis elegans (C. elegans) model. In addition, the situation of lipid depositon in C. elegans was observed by oil red O staining, TG concentration and LPS activity were measured to explore their lipid-lowering potential. The results showed that the alcohol extract of G. frondosa was mainly an active protein, the content was 233.67 mg/g. It had significant scavenging ability on DPPH, ABTS, and hydroxyl radicals, and the IC50 values were 3.25 mg/mL, 0.38 mg/mL, and 1.73 mg/mL, respectively. Compared with the blank group, the activities of CAT, T-SOD, GSH-Px, and LPS in the high-dose group were significantly increased by 4.33, 4.88, 2.28, and 3.93 times, respectively. The levels of fat deposition, MDA, and TG decreased significantly, and the levels of MDA and TG in the high-dose group decreased by 55.62% and 50.66%, respectively. In conclusion, the alcohol extract of Grifola frondose has antioxidant capacity in vitro and can enhance the antioxidant enzyme activity in C. elegans. The lipid deposition level and TG concentration in C. elegans were decreased, indicating that it had a good lipid lowering effect. This study can provide a theoretical basis for the high-value utilization of Grifola frondose.