通过建立慢性轻度不可知应激刺激（CUMS）诱导的小鼠抑郁模型，研究当归挥发油对抑郁行为的影响及机制。小鼠随机分为正常组、模型组、低、中、高剂量当归挥发油组（15、30、60 mg/kg）及盐酸氟西汀组（2.1 mg/kg）。干预4 周后发现，与模型组比较，当归挥发油各剂量组小鼠中央穿越次数、中央区活动时间及糖水偏好度升高（P<0.01），悬尾不动时间下降（P<0.05，P<0.01），海马CA1 区病理变化减轻，血清白细胞介素-1β（IL-1β）、IL-6 及肿瘤坏死因子-α（TNF-α）含量降低（P<0.01），而IL-10 含量增加（P<0.01）；除低剂量当归挥发油组小鼠脑组织多巴胺（DA）含量及神经营养蛋白-3（NT-3）表达无明显变化外（P>0.05），剩余各组小鼠DA、去甲肾上腺素（NE）、5- 羟色胺（5-HT）含量及神经生长因子（NGF）、NT-3、脑源性神经营养因子（BDNF）表达均升高（P<0.05，P<0.01）。上述结果表明，当归挥发油具有改善CUMS 诱导的小鼠抑郁行为作用，该作用与抑制神经炎症、上调单胺类神经递质含量及促进神经营养因子表达有关。

To determine the effects and mechanism of Angelica sinensis volatile oil (ASVO) on depressive behavior, chronic unpredictable mild stress (CUMS)-induced depression mouse models were established. Mice were divided into normal, model, low-dose, medium-dose, and high-dose ASVO (15, 30, and 60 mg/kg, respectively), and fluoxetine hydrochloride (2.1 mg/kg) groups. After four weeks of intervention, the number of central zone crossing, central zone activity duration, and sucrose preference were increased (P<0.01) while the tail suspension time was reduced (P<0.05, P<0.01) in all ASVO groups compared with those in the model group. The pathological changes in the hippocampal CA1 region were alleviated, and the contents of serum interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) decreased (P<0.01) while that of IL-10 increased (P<0.01). Despite no significant changes in the dopamine (DA) content and neurotrophin-3 (NT-3) expression in the brain tissue of mice in the low-dose ASVO group (P>0.05), the contents of DA, norepinephrine (NE), and 5-hydroxytryptamine (5-HT) and the expression of nerve growth factor (NGF), NT-3, and brain-derived neurotrophic factor (BDNF) increased in the other groups (P<0.05, P<0.01). These results indicate that ASVO can improve CUMS-induced depressive behavior in mice, which is related to the inhibition of neuroinflammation, upregulation of monoamine neurotransmitter contents, and promotion of neurotrophic factor expression.

吉林省教育厅科技项目（JJKH20210059KJ ；JJKH20210055KJ）