荞麦槲皮素对高脂饮食大鼠脂代谢及脂肪因子的调节作用

Regulatory Effects of Buckwheat Quercetin on Lipid Metabolism and Adipokines in Rats Receiving A High-fat Diet

摘要:

该文研究了荞麦槲皮素（QYE）对高脂饮食大鼠脂质代谢及脂肪因子的影响。将54只SD大鼠随机分为对照组、模型组、阳性组、荞麦槲皮素低（50 mg/kg）、中（100 mg/kg）、高（200 mg/kg）剂量组，建立高脂血症大鼠模型5周后，灌胃荞麦槲皮素干预治疗，测定各组大鼠血清、肝脏脂质水平，以及血清抗氧化、脂肪因子水平，并观察肝脏组织病理切片。结果表明，与模型组相比，荞麦槲皮素能够降低血清和肝脏TC、TG的含量，其中QUE高剂量组血清中TC、TG含量分别下降了33.47%和28.66%，较模型组差异极显著（P<0.01）；荞麦槲皮素高剂量组的Chemerin水平下降12.3%，CMKLR1水平下降19.83%，TLR4水平下降15.87%，NF-κB水平下降14.12%。同时荞麦槲皮素高剂量组上调了血清中33.87 U/mL的GSH-Px酶活力，差异极显著（P<0.01），荞麦槲皮素高剂量组下调了血清中79.56 U/mL的CHE含量。由此可见，荞麦槲皮素能显著调节高脂膳食大鼠血脂异常，降低Chemerin及其受体CMKLR1、TLR4、水平，抑制NF-κB通路活化，减少胆固醇堆积，能够调节脂质代谢及脂肪因子的水平。

Abstract:

To examine the effects of buckwheat quercetin on lipid metabolism and adipokines in rats receiving a high-fat diet, 54 SD rats were randomly divided into a control group; a model group; a positive group; and groups receiving low (50 mg/kg), moderate (100 mg/kg), and high (200 mg/kg) doses of buckwheat quercetin (QUE). Five weeks after establishing a hyperlipidemic rat model, buckwheat quercetin was administered to the rats as intervention. Serum and liver lipid levels as well as serum antioxidant and adipokine levels of rats in each group were measured, and histopathological sections of their livers were examined. Compared with those in the model group, serum and liver TC and TG levels were significantly reduced in groups receiving QUE; specifically, the high-dose QUE group experienced a 33.47% and 28.66% decrease in serum TC and TG levels, respectively, indicating significant differences compared with values in the model group (P<0.01). Moreover, the high-dose QUE group experienced a 12.3% decrease in chemerin levels, 19.83% decrease in CMKLR1 levels, 15.87% decrease in TLR4 levels, and 14.12% decrease in NF-κB levels; simultaneously, however, serum GSH-Px activity was increased by 33.87 U/mL, which was extremely significant (P<0.01), whereas serum CHE activity was decreased by 79.56 U/mL in the high-dose QUE group. Thus, buckwheat quercetin closely regulated dyslipidemia in rats receiving a high-fat diet, reduced the levels of chemerin and its receptors CMKLR1 and TLR4, inhibited the activation of the NF-κB pathway, suppressed the accumulation of cholesterol, and regulated the metabolism of lipids and levels of adipokines.