为评价不同非热杀菌方式对果蔬原料杀菌效果及品质的影响，该研究分别采用超高压技术（High Hydrostatic Pressure，HHP）、低温等离子体技术（Cold Plasma，CP）和辐照技术三种方式对百香果鲜榨果浆进行杀菌处理。结果表明，三种非热处理方式对果浆中的微生物（2.15 lg(CFU/g)、2.44 lg(CFU/g)、2.34 lg(CFU/g)）均有一定的抑制和杀灭作用，其中300 MPa及以上压力处理和3 kGy辐照量处理后果浆的菌落数均＜1 lg(CFU/g)，这两种方式对百香果果浆均有较好的杀菌效果。为了进一步探究超高压处理对果浆贮藏期的影响，该研究还探讨了超高压处理前后的果浆在-20 ℃的条件下储存9个月内微生物含量的变化，实验得出，500 MPa和600 MPa处理的百香果果浆在-20 ℃下储存9个月后微生物含量仍＜2 lg(CFU/g)，符合国家安全规定。在理化性质方面，超高压技术和低温等离子体技术能有效保持果浆的色泽（ΔE＜2）和风味（p＞0.05），且能抑制果浆POD酶、PPO酶的活性，但辐照处理无法钝化PPO酶。综上所述，超高压技术能有效杀灭百香果果浆中的菌落，并能更好地保持果浆的品质，因此超高压技术在百香果果浆中具有较好的商业应用前景。

To evaluate the influences of different non-thermal methods on the sterilization effectiveness and quality of raw fruit and vegetable materials, high hydrostatic pressure (HHP) technology, cold plasma (CP) technology, and irradiation technology were applied to sterilize freshly pressed passion fruit pulp. The results indicated that the three non-thermal treatments could inhibit and kill to certain extents the microorganisms in the pulp (2.15 lg(CFU/g), 2.44 lg(CFU/g), 2.34 lg(CFU/g), respectively). The number of colonies in the pulp was lower than 1 lg (CFU/g) after the treatment at a pressure of 300 MPa and above and the treatment with an irradiation dose of 3 kGy, indicating that both methods had a good sterilizing effect on passion fruit pulp. In order to explore further the effect of HHP treatment on the storage period of fruit pulp, this study also investigated the changes in the microbial contents of fruit pulps with and without a high hydrostatic pressure treatment during the storage at -18 °C for 9 months. The experiments showed that the microbial contents of the passion fruit pulps treated at 500 MPa and 600 MPa were still in compliance with the National Safety Regulation after being stored at -20 °C for 9 months (both<2 lg (CFU/g)). In terms of physico-chemical characteristics, the HHP technology and CP technoldogy coul effectively retain the color (ΔE<2) and flavor (p>0.05) of the fruit pulp, and exerted certain inhibitory effects on the activities of POD and PPO enzymes, although the irradiation treatment could not inactivate the PPO enzyme. In summary, the HHP technology can effectively kill the microbiam colonies in the passion fruit pulp while retaining better the quality of the pulp. Therefore, HHP technology has good commercial application prospects in processing passion fruit pulp.

广东省重点研发计划项目（2019B020212004；2020B020225002）；中国工程物理研究院中子物理学重点实验室开放基金（2019DB01）