该研究采用不同总硒浓度的富硒平菇粉联合5-FU处理肝癌细胞HepG2，应用CCK-8法、流式细胞术双染法探讨富硒平菇粉联合5-FU的抑癌效果，并以富硒平菇粉为原料研制一款富硒代糖面包，为富硒功能食品研发提供参考依据。结果表明，富硒平菇粉对肝癌细胞HepG2增殖有抑制作用，抑制率范围为13.89%~27.39%；不同总硒浓度的富硒平菇粉联合5-FU干预HepG2肝癌细胞36 h后，凋亡细胞数量呈硒作用浓度依赖性增高（p<0.05）；总凋亡率分别是26.36%~49.72%，且凋亡率高于5-FU组（p<0.05)。富硒代糖面包的最佳加工工艺配方为：200 g面粉中富硒平菇粉添加量为0.06 g，复配代糖添加量为25 g，烘烤温度为上下火175 ℃，烘烤时间为20 min，总硒含量为（0.17±0.01）mg/kg。综上所述，采用不同总硒浓度的富硒平菇粉联合5-FU处理肝癌细胞HepG2，发现富硒平菇粉联合5-FU可抑制HepG2肝癌细胞生长，起到增效解毒的作用，并成功研制出富硒代糖面包的最佳工艺，具有较好的研究和应用价值。

Selenium (Se)-enriched Pleurotus ostreatus fungus powder with different total Se concentrations was used in combination with 5-FU to treat HepG2 hepatocellular carcinoma cells. The CCK-8 and flow cytometry double staining methods were applied to investigate the tumor suppressive effect of Se-enriched P. ostreatus fungus powder combined with 5-FU. The Se-enriched P. ostreatus fungus powder was then used as the raw material to develop a Se-enriched sugar substitute bread, providing scientific evidence for the development of Se-enriched functional foods. The results demonstrate that the Se-enriched P. ostreatus fungus powder had an inhibitory effect on the proliferation of HepG2 hepatocellular carcinoma cells, with the inhibition rate ranging from 13.89% to 27.39%. The number of apoptotic cells in HepG2 hepatocellular carcinoma increased in a concentration-dependent manner after treatment with different total Se concentrations of Se-rich P. ostreatus fungus powder combined with 5-FU for 36 h (p<0.05). The total apoptosis rates were 26.36%~49.72%, respectively, and the apoptosis rates were higher than those in the 5-FU group (p<0.05). The optimal processing formula of Se-enriched sugar-substitute bread was as follows: 0.06 g Se-enriched P. ostreatus fungus powder was added to 200 g flour with 25 g compound sugar substitute and baked at 175 °C for 20 min; the total Se content was 0.174±0.011 mg/kg. In conclusion, when HepG2 hepatoma cells were treated with Se-enriched P. ostreatus fungus powder at different total Se concentrations combined with 5-FU, HepG2 hepatoma cell growth was inhibited, increasing the efficiency of detoxification. The optimal processing formula of Se-enriched sugar substitute bread, which has various research and application values, was also developed.

国家自然科学基金面上项目（81773428）