该研究对比探讨了苯酚-硫酸法、蒽酮-硫酸法、硫酸-紫外法、二硝基水杨酸法测定藤茶中多糖含量的准确性，利用单因素试验对4种方法的关键检测条件进行了优化，并对方法学的精密度、重复性、稳定性、加标回收率进行了比较，再用最佳实验条件检测藤茶样品中多糖的含量。结果表明，用于藤茶多糖测定的最佳方法为苯酚-硫酸法，其最适紫外检测波长为485.5 nm，以葡萄糖为标准在0.1~0.6 mg/mL范围内吸光度具有良好的线性关系。在0.5 mL藤茶粗多糖溶液（质量浓度为0.5 mg/mL）反应体系中，在500 μL浓硫酸、600 μL 5wt%苯酚溶液、60 ℃水浴温度和20 min水浴时间的最佳实验条件下，测得其多糖含量为59.43%，其精密度最高（RSD 0.18%），稳定性最好（RSD 0.30%）。最后测得藤茶中幼嫩茎叶和粗老茎叶的多糖含量为9.87~10.47 mg/g和29.77~30.47 mg/g。

The accuracy of the phenol-sulfuric acid method, the anthrone-sulfuric method, the sulfuric acid-UV method, and the dinitrosalicylicacid colorimetric method for the determination of the polysaccharides in vine tea (Ampelopsis grossedentata) was investigated. The key detection conditions of the four methods were optimized by the single factor test. The accuracy, repeatability, stability, recovery rates of the four methods were compared with the optimal experimental conditions for the determination of polysaccharides in vine tea samples. The results showed that the phenol-sulfuric acid method was the most effective for the determination of polysaccharides in vine tea. The optimal UV detection wavelength was 485.5 nm, and the absorbance at 0.1~0.6 mg/mL with glucose as the standard had a good linear relationship. Under the optimal conditions of 500 μL concentrated sulfuric acid, 600 μL 5% phenol solution, 60 ℃ water bath temperature, and 20 min water bath time in 0.5 mL crude polysaccharide solution (concentration 0.5 mg/mL) of vine tea, the phenol-sulfuric acid method exhibited the highest precision (RSD 0.18%) and the best stability (RSD 0.30%). The results showed that the polysaccharide content of young and old stems and leaves was 9.87~10.47 and 29.77~30.47 mg/g, respectively.

广东省科技计划项目（2021B0202060001）；粤财农〔2021〕170号；广州市科技计划项目（202206010079）