[关键词]
[摘要]
该研究旨在探究在模拟物流条件下蓝莓、树莓和接骨木莓提取物多酚含量及其抗氧化活性的变化,采用循环变温法模拟陆地物流运输条件[30 ℃(7 h)→35 ℃(6 h)→45 ℃(5 h)→55 ℃(5 h)→60 ℃(1 h)→30 ℃(7 h)],Folin酚法测定不同物流时间下浆果提取物多酚含量,2,2-联苯基-1-苦基肼基(2,2-Diphenyl-1-picrylhydrazyl,DPPH·)清除活性和氧自由基吸收能力(Oxygen Radical Absorbance Capacity,ORAC)评价不同物流时间下浆果提取物抗氧化活性的变化。结果表明,接骨木莓提取物、蓝莓提取物和树莓提取物初始多酚质量分数分别为14.96%、5.11%和2.53%,随着循环时间的积累,仅接骨木莓提取物多酚含量显著升高;三者DPPH·清除活性均降低,总IC50增长率均超过20%;接骨木莓提取物、树莓提取物和蓝莓提取物ORAC波动较大,其Trolox当量分别下降62.76%、32.74%和19.72%。模拟物流温度变化与时间积累会影响蓝莓提取物、树莓提取物和接骨木莓提取物抗氧化活性,DPPH·清除活性稳定性依次为树莓<蓝莓<接骨木莓,ORAC稳定性依次为蓝莓<接骨木莓<树莓。
[Key word]
[Abstract]
A cyclic temperature change model was used to simulate land transportation conditions [30 ℃ (7 h) → 35 ℃ (6 h) → 45 ℃ (5 h) → 55 ℃ (5 h) → 60 ℃ (1 h) → 30 ℃ (7 h)] to investigate the effects of logistics and transportation on the polyphenol contents and antioxidant activities of blueberry, raspberry, and elderberry extracts. The folin-phenol method was used to determine the polyphenol contents in the three berry extracts under different transportation times. Additionally, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and oxygen radical absorption capacity (ORAC) were used to evaluate the antioxidant activities of the three extracts under different transportation times. The results demonstrate that the initial polyphenol contents of the elderberry, blueberry, and raspberry extracts were 14.96%, 5.11%, and 2.53%, respectively. As the transportation time increased, only the polyphenol content of the elderberry extract increased significantly. Over time, the DPPH· scavenging activities and ORAC of all the three extracts decreased, and the total increased rates of IC50 were all higher than 20%. It is worth noting that the ORAC for all three extracts showed remarkable fluctuations. The total trolox equivalent loss rates of the elderberry, raspberry, and blueberry extracts were 62.76%, 32.74% and 19.72% respectively. The temperature changes and transportation duration times under logistics simulation affected the antioxidant activities of all three berry extracts. The stabilities of the DPPH scavenging activities in ascending order were: raspberry < blueberry < elderberry, while the stabilities of ORAC was blueberry < elderberry < raspberry.
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[基金项目]
广东省国际科技合作项目专题(2022A0505050012);“一带一路”创新人才交流外国专家项目(DL2022163004L)