探讨不同海拔刺梨原汁对2型糖尿病（Type 2 Diabetes Mellitus，T2DM）小鼠糖脂代谢紊乱及氧化损伤的治疗效果。采用高脂高糖联合链脲佐菌素（Streptozotocin，STZ）诱导T2DM小鼠模型，设置空白组（NC）、模型组（MC）、刺梨原汁高（HF）、中（MF）、低海拔（DF）组及对照组（PC），每组10只，干预28 d，期间测定小鼠摄食量、饮水、体质量和空腹血糖值（FBG），解剖后测定糖耐量、糖化血清蛋白（GSP）、血红蛋白（GHb）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及谷胱甘肽酶（GSH-Px），并通过HE染色观察各组小鼠肝脏组织结构。结果表明，不同海拔刺梨原汁可降低FBG、GSP、GHb、TG、TC、LDL-C浓度及MDA含量，提高CAT、SOD、GSH-Px活性及HDL-C浓度；其中HF组肝脏CAT、SOD、GSH-Px活性比MC组提高了151.27%、63.79%、67.46%。HE染色结果显示，不同海拔刺梨原汁组肝组织损伤均有所缓解。结果说明，不同海拔刺梨原汁对T2DM小鼠引发的糖脂代谢紊乱和氧化应激稳态具有一定的修复作用。

To investigate the therapeutic effects of Rosa roxburghii juice from different altitudes on the glycolipid metabolism and oxidative damages in mice with type 2 diabetes mellitus (T2DM), T2DM mouse models were established using high fat and high sugar diets combined with streptozotocin (STZ). The mice were divided into the blank group (NC), the model group (MC), the high-altitude R. roxburghii juice-treated group (HF), the medium-altitude R. roxburghii juice-treated group (MF), the low-altitude R. roxburghii juice-treated group (DF) and the control group (PC), each of which contained 10 mice. The feed intake, water intake, body weight, and fasting blood glucose (FBG) of the mice were measured during the 28 day intervention, which was followed by dissection to measure the glucose tolerance and the glycosylated serum protein (GSP) and glycosylated hemoglobin (GHb) contents. In addition, the total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) contents in the serum, kidney and liver were determined. The liver tissues from each group were observed by hematoxylin & eosin (HE) staining. The results show that all-altitude R. roxburghii juice reduces the FBG, GSP, GHb, TG, TC, LDL-C, and MDA contents, but increases the CAT, SOD, and GSH-Px activities and the HDL-C level. Compared with those in the MC group, the liver CAT, SOD, and GSH-Px activities in the HF group increase by 151.27%, 63.79%, and 67.46% respectively. HE staining results demonstrate that liver tissue damages are alleviated in all R. roxburghii juice-treated groups. The results indicate that Rosa roxburghii juice from all altitudes can, to a certain degree, improve glycolipid metabolism disorder and oxidative stress homeostasis in T2DM mice.

贵州省林业局科技项目（黔林科合[2020]2011）；贵州省工业与信息化厅专项（黔财农[2020]307）