该研究在小鼠膳食中添加三种不同的益生元（菊粉、低聚果糖、壳寡糖），探究其对虾青素消化吸收的影响。结果表明，菊粉、壳寡糖对于藻源虾青素的生物可接受率无显著影响（p>0.05），低聚果糖显著降低了虾青素的生物可接受率（p<0.01）。膳食中添加菊粉可以适当提高藻源虾青素的生物利用度，与对照组相比，菊粉组小鼠肝脏中的虾青素代谢曲线下面积（Area Under Curve，AUC）显著提高了36.37%。菊粉组小鼠粪便中短链脂肪酸（Short Chain Fatty Acids，SCFAs）的含量显著升高（p<0.05），其中乙酸的含量升高了139.54%，丙酸的含量升高了40.04%。16S rRNA扩增子测序结果表明，菊粉组的杜氏杆菌属、阿克曼氏菌属的相对丰度分别提高了70.55%和83.33%。综上，膳食中添加菊粉可能通过改变肠道菌群的结构来改善藻源虾青素在机体内的吸收利用。本研究成果为提高虾青素的生物利用度提供了新思路，为虾青素产品的开发提供了科技支撑。

Three different prebiotics (inulin, fructose-oligosaccharide, and oligo-chitosan) were added to the diets of mice to explore their effects on the digestion and absorption of astaxanthin. The results showed that inulin and oligo-chitosan had no significant effects on the bioaccessibility of algal-derived astaxanthin (p>0.05), whereas fructose-oligosaccharide reduced its bioaccessibility (p<0.01). The bioavailability of algal-derived astaxanthin could be reasonably improved with inulin supplementation. In comparison with the control group, the inulin-treated group showed a significant increase of 36.37% in the area under curve (AUC) of astaxanthin in the liver. The content of short-chain fatty acids (SCFAs) also significantly increased (p<0.05) in the feces of the mice from the inulin-treated group. In particular, the level of acetic acid increased by 139.54% and that of propionic acid increased by 40.04%. 16SrRNA amplicon sequencing results indicated that the relative abundance of Dubosiella and Akkermansia in the insulin-treated group increased by 70.55% and 83.33%, respectively. In conclusion, dietary inulin supplementation may improve the absorption and utilization of algal-derived astaxanthin in the body by changing the composition of intestinal flora. The results of this study highlight a new idea for improving the bioavailability of astaxanthin and provide scientific and technological support for the development of astaxanthin products.

国家重点研发计划项目（2018YFC0311201）