该文研究了茄皮提取物（Solanum melongena Peels Extract，SMPE）对糖尿病小鼠的降血糖作用，采用LC-QTOF-MS阐明茄皮提取物的物质基础。体外实验采用不同浓度SMPE和阳性对照药阿卡波糖进行α-葡萄糖苷酶活性抑制试验，测定抑制率。体内试验采用四氧嘧啶对小鼠进行腹腔注射，建立糖尿病小鼠模型。将实验小鼠分为7组：正常对照组、正常给药组（正常小鼠给予15 g/kg SMPE）、SMPE高剂量组（15 g/kg）、SMPE中剂量组（5 g/kg）、SMPE低剂量组（2.5 g/kg）、阳性药组（阿卡波糖1 g/kg）、模型组。连续灌胃4周，并观察小鼠体质量、空腹血糖浓度和糖耐量。结果表明，SMPE 10 mg/mL对α-葡萄糖苷酶抑制率达49.13%，且不同浓度SMPE对α-葡萄糖苷酶的抑制作用呈剂量依赖性；SMPE对正常小鼠的体质量、空腹血糖浓度无影响；与模型组相比，SMPE低、中、高剂量组体质量分别增加了20.34%、23.91%和15.20%，空腹血糖浓度分别下降了5.97%、11.91%和42.20%。SMPE能够显著降低糖尿病小鼠的血糖浓度，其机制可能与SMPE抑制α-葡萄糖苷酶活性、延迟肠道对葡萄糖的吸收有关。

The hypoglycemic effect of Solanum melongena peels extract (SMPE) on diabetic mice was investigated. LC-QTOF-MS was employed to clarify the material basis of SMPE. Different concentrations of SMPE, and the positive control, the drug acarbose, were used for in vitro experiments to conduct the α-glucosidase activity inhibition tests to determine the inhibition rate. In in vivo experiments, the diabetic mouse model was established through injecting alloxan intraperitoneally into mice, and the experimental mice were divided into 7 groups: the control group, normal administration group (normal mice were given 15 g/kg SMPE), SMPE high-dose group (SMPE 15 g/kg), SMPE medium-dose group (SMPE 5 g/kg), SMPE low-dose group (SMPE 2.5 g/kg), positive drug group (acarbose 1 g/kg), and model group. After continuous intragastric gavage for 4 weeks, the body weight, fasting blood glucose level, and glucose tolerance were measured. The results showed that the α-glucosidase inhibitory rate of 10 mg/mL SMPE was 49.13%, and SMPE at different concentrations inhibited α-glucosidase in a dose-dependent manner. SMPE had no effect on body weight and fasting blood glucose in normal mice. Compared with the model group, the body weights of the SMPE low-, medium-, and high-dose groups increased by 20.34%, 23.91%, and 15.20%, respectively, and the fasting blood glucose values decreased by 5.97%, 11.91%, and 42.20%, respectively. SMPE exhibited a significant hypoglycemic effect in diabetic mice, and the underlying mechanism may be related to the abilities of SMPE to inhibit the activity of α-glucosidase and delay the intestinal absorption of glucose.

国家自然科学基金资助项目（32101957）；中国农业科学院创新工程（1125161015000150013）