Abstract:In order to develop new and safe compound biological preservative for Agaricus bisporus, 3 categories and 12 kinds of common biological preservative were selected through the optimal screening experiment, and the preservative with better protective effect on the whiteness, opening degree, texture, hair viscosity and smell of Agaricus bisporus was screened out. The effects of ascorbic acid (Vc), Nisin and carrageenan on the preservation of Agaricus bisporus after storage were investigated. After screening, it was found that Vc, Nisin and carrageenan had a good effect on delaying the Browning of Agaricus bisporus. Then the optimization results showed that the compound biological preservative prepared by Vc 0.80 g/L, Nisin 0.40 g/L and carrageenan 5.40 g/L had the best effect. The whiteness value at the end of storage was 21.32% higher than that of the negative control (CK). Compared with the control group, the compound biological preservative treatment significantly reduced the weight loss rate and decay rate of Agaricus bisporus during storage, inhibited the decline of hardness, delayed the enhancement of polyphenol oxidase (PPO) activity, accelerated the peak of total phenol content and promoted the accumulation of phenols, promoted the peak of ABTS cation free radical clearance, and effectively delayed the reduction of antioxidant capacity of Agaricus bisporus. The results showed that the Vc-Nisin-carrageenan compound biological preservative had a good protective effect on the storage quality of Agaricus bisporus. This study can provide reference and basis for developing new, safe and efficient compound biological preservative of Agaricus bisporus.
