基于高通量测序探究东北粳稻储藏期间真菌群落的演替

High-throughput sequencing-based analysis of the succession of fungal communities during the storage of northeast japonica rice

摘要:

为探究不同初始水分的东北粳稻在模拟实仓环境储藏的条件下，稻谷中真菌群落随储藏时间的演替变化。选取水分在14.50%~15.50%的稻谷HW和13.50%~14.50%稻谷LW，进入模拟储藏实验。结果表明，在第一和第二储藏期高水分稻谷HW水分含量由15.50%降低至15.09%，低水分稻谷LW水分含量由14.21%降低至13.59%，直至第三储藏期保持平稳。基于可操作性分类单元（Operational Taxonmic Units，OTUs）的物种分类分析，两种水分的稻谷真菌群落经历了不同的演替变化。高水分稻谷在每个储藏期，真菌群落都发生了明显演替，稻瘟病菌（Magnaporthe_grisea）、金黄色蝶形担孢酵母（Papiliotrema_aurea）、弯孢菌（Curvularia_inaequalis）、黑孢霉（Neosetophoma_samararum）、汉纳酵母菌（Hannaella_sinensis）在储存结束时成为了最终的优势菌种。低水分稻谷在第二储藏期真菌群落发生明显演替，优势菌种为弯孢菌（Curvularia_inaequalis）、汉纳酵母菌（Hannaella_sinensis）、Hannaella_zeae。

Abstract:

To explore the succession of the fungal communities of the northeast japonica rice with different initial moistures under the conditions simulating those of the real storage ware house environment. The rice with a moisture content of 14.50%~15.50%, HW, and the rice with a moisture content of 13.50%~14.50%, LW, were sued for the simulated storage experiments. The results showed that the moisture contents of the HW rice and LW rice decreased from 15.50% to 15.09% and from 14.21% to 13.59%, respectively, in the first and second storage periods, and remained stable in the third storage period. According to the taxonomic analysis based on operational taxonomic units (OTUs), the fungal communities of the two types of rice underwent different successional changes. The fungal commnity of the HW rice exhibited obvious succession during each storage period, and Magnaporthe_grisea, Papiliotrema_aurea, Curvularia_inaequalis, Neosetophoma_samararum, Hannaella_sinensis became the final dominant species at the end of storage. The fungal community of the LW rice experienced obvious succession in the second storage, Curvularia_inaequalis, Hannaella_sinensis and Hannaella_zeae became the dominant species.