该研究探讨了姜黄植物饮料（以下简称“姜黄饮”）对KM小鼠的解酒作用及可能的作用机制。构建高浓度酒精致小鼠醉酒模型，通过小鼠防醉试验行为学变化、醉酒小鼠血液乙醇浓度、体内乙醇代谢关键酶的含量或活性及胃肠组织的变化，评价姜黄饮对小鼠的解酒作用。结果显示，姜黄饮高剂量组醉酒潜伏期为235.00 min，与模型组相比显著延长（p<0.05）；醒酒时间为232.00 min，与模型组相比显著缩短（p<0.05）。血液乙醇含量为4.21 mg/mL，与模型组相比极显著降低（p<0.01）；乙醇脱氢酶、乙醛脱氢酶活力分别为3.74 U/mg prot和8.36 U/mg prot，与模型组相比显著提高（p<0.05）；辅酶Ⅰ和还原型辅酶Ⅰ含量分别为0.08 nmol/mg prot和0.39 U/mg prot，NADH/NAD+比值与模型组相比极显著提高（p<0.01）；细胞色素P450、谷胱甘肽过氧化物酶含量分别为78.51 pg/mg和1341.00 pg/mg，与模型组相比极显著提高（p<0.01）。胃部、肠组织观察和病理切片结果显示，姜黄饮低、高剂量组可减轻乙醇对小鼠肠道引起的损伤，减少小鼠肠道出血和水肿。上述结果表明，姜黄饮对醉酒小鼠有明显的解酒作用，其作用机制可能与其增强机体乙醇代谢路径关键酶及抗氧化酶的活性，加快体内乙醇代谢速度，保护肝脏及胃肠道有关。

The antialcoholism effect and possible mechanism of curcuma botanical beverage in KM mice were investigated in this paper. After establishing the model of high concentration alcohol-induced intoxication in mice, the anti-alcoholism effect of curcuma botanical beverage were evaluated according to the behavior of anti-inebriation test in mice, the concentration of ethanol in blood, the content or activity of the key enzyme of ethanol metabolism in vivo, and the changes of gastrointestinal tissue. The results showed that the latency of drunkenness was 235.00 min, which was significantly longer than that in the model group (p<0.05). The time required to regain consciousness was 232.00 min, which was significantly shorter than that in the model group (p<0.05). The concentration of ethanol in the blood was 4.21 mg/mL, which was significantly lower than that of the model group (p<0.01). The activities of alcohol dehydrogenase and acetaldehyde dehydrogenase were 3.74 U/mg prot and 8.36 U/mg prot, respectively, which were significantly higher than those of the model group (p<0.05). The contents of coenzyme I and reduced coenzyme I were 0.08 nmol/mg prot and 0.39 U/mg prot, respectively. The ratio of NADH to NAD+ was significantly higher than that of model group (p<0.01). The contents of cytochrome P450 and glutathione peroxidase were 78.51 pg/mg and 1341.00 pg/mg, respectively, which were significantly higher than those in the model group (p<0.01). The results of stomach and intestinal tissue observation and pathological section showed that low and high dosage of curcuma botanical beverage could alleviate the injury of intestinal tract caused by ethanol and reduce the intestinal bleeding and edema. The above results indicated that curcuma botanical beverage has obvious anti-alcoholism effect on drunken mice. The mechanism may be related to enhancing the activities of key enzymes and antioxidant enzymes in the pathway of ethanol metabolism, speeding up the metabolism of ethanol in vivo, and protecting the liver and gastrointestinal tract.

广州开发区创新领军人才项目（2020-l010）；肇庆高新区创新团队项目（2019ZHQ10）