该研究制备了性状稳定的纳米硒，并对其进行表征；并分析了不同浓度纳米硒对双孢菇子实体中硒含量、水分、粗多糖、总氮、粗脂肪、粗纤维、总游离氨基酸、总氨基酸含量及双孢菇单朵均重的影响；以及培养基中添加不同浓度纳米硒双孢菇子实体的综合品质。结果表明，除水分和硒含量随着纳米硒浓度的增加而上升，其他成分含量均随着培养基中纳米硒含量的增加呈现先上升后下降的趋势。双孢菇培养基中纳米硒添加量为0.5~2.5 mg/kg时，双孢菇子实体中硒含量达到0.19~0.90 mg/kg干物质，比对照双孢菇中硒含量提高2.38~11.25倍。聚类分析和主成分分析结果均表明培养基中纳米硒添加量为1.0 mg/kg时，双孢菇子实体的综合品质最佳，单朵均重达到22.90 g，保藏期约延长1~2 d。上述研究结果可为富硒双孢菇的深入研究以及其大规模生产提供数据支撑。

In this study, nano-selenium with stable properties was prepared and characterized; The effects of different concentrations of nano- selenium on selenium content, water content, crude polysaccharide, total nitrogen, crude fat, crude fiber, total free amino acid, total amino acid content and average weight of single flower of Agaricus bisporus were analyzed; And the comprehensive quality of fruiting bodies of Agaricus bisporus with different concentrations of nano-selenium in the culture medium was also explored. The results showed that the content of other components increased first and then decreased with the increase of nano-selenium content in the culture medium, except that the content of water and selenium increased with the increase of nano-selenium concentration. When the amount of nano-selenium in Agaricus bisporus medium was 0.5~2.5 mg/kg, the selenium content in fruiting body of Agaricus bisporus reached 0.19~0.90 mg/kg dry matter, which was 2.38~11.25 times higher than that in the control. Cluster analysis and principal component analysis showed that the comprehensive quality of fruiting body of Agaricus bisporus was the best, the average weight of single flower reached 22.90 g, and the storage period could be prolonged for 1~2 d when the optimal addition amount of nano-selenium in the culture medium was 1.0 mg/kg. The above results can provide data support for the in-depth study of selenium-enriched Agaricus bisporus and its large-scale production.

国家自然科学基金项目（31301537）；中山市科技专项（2018A1007）；阳西市科技创新战略专项（SDZX2021030）