桑叶生物碱对小鼠酒精性肝损伤的保护作用

Ameliorative Effect of Mulberry Leaf Alkaloids on Alcoholic Liver Injury in Mice

摘要:

探讨桑叶生物碱（mulberry leaf alkaloids，MLA）对酒精性肝损伤小鼠的改善作用。建立酒精性肝损伤小鼠模型，以MLA（40、80、160 mg/kg•day）干预4周，计算肝脏指数，检测血清甘油三酯（TG）、总胆固醇（TC）、谷丙转氨酶（ALT）、谷草转氨酶（AST）和肝脏中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、总超氧化物歧化酶（SOD）、丙二醛（MDA）、肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、白细胞介素6（IL-6）的含量。与模型组相比，MLA中剂量组小鼠的肝脏指数、血清TG、TC、ALT、AST和肝脏MDA、IL-1β、IL-6、TNF-α水平分别降低11.18%、22.78%、19.19%、28.08%、16.16%、25.07%、23.51%、18.61%、11.78%，肝脏SOD、CAT、GSH-Px活力分别提高27.47%、15.36%、27.83%；MLA高剂量组小鼠的肝脏指数、血清TG、TC、ALT、AST和肝脏MDA、IL-1β、IL-6、TNF-α水平分别降低15.17%、32.65%、23.16%、30.58%、16.41%、31.97%、27.19%、25.41%、29.59%，肝脏SOD、CAT、GSH-Px活力分别提高38.38%、19.09%、31.09%；MLA中、高剂量组肝脏组织结构损伤明显改善。MLA可以改善小鼠酒精性肝损伤，其作用机制可能与改善肝脏氧化应激和抑制炎症反应有关。

Abstract:

Mulberry leaf alkaloids (MLA) were tested for their ability to ameliorate alcoholic liver injury (ALI) in a mouse model of the disease. First, ALI was induced in mice, following which the animals were treated with one of three different doses of MLA (40, 80, and 160 mg/kg•day) for four weeks. Then, the serum levels of triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST); enzymatic activities of liver catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD); and liver levels of malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and interleukin-6 (IL-6) were measured. Compared with the values from the control group, the liver index; serum levels of TG, TC, ALT, and AST; and MDA, IL-1β, IL-6, and TNF-α levels of the group treated with middle-dose MLA were lower by 11.18%, 22.78%, 19.19%, 28.08%, 16.16%, 25.07%, 23.51%, 18.61%, and 11.78%, respectively. By contrast, the liver SOD, CAT, and GSH-Px activities of the middle-dose MLA group were higher by 27.47%, 15.36%, and 27.83% respectively. In the high-dose MLA group, the liver index; serum levels of TG, TC, ALT, and AST; and liver levels of MDA, IL-1β, IL-6, and TNF-α were lower by 15.17%, 32.65%, 23.16%, 30.58%, 16.41%, 31.97%, 27.19%, 25.41%, and 29.59%, respectively, than the levels in the control group; however, the liver SOD, CAT, and GSH-Px activities were higher by 38.38%, 19.09%, and 31.09%, respectively. The structural damage to the liver tissue was significantly ameliorated in the medium- and high-dose MLA groups. In summary, MLA can ameliorate alcoholic liver injury in mice, with the possible mechanism being related to its actions in alleviating liver oxidative stress and inhibiting the inflammatory response.