Abstract:In this study, the grape seeds and artichoke leaves subjected to different crushing treatments were added to the culture medium, and the effects of industrial by-products on the nutrients and active components of Pleurotus ostreatus were mainly investigated. Using total phenolic content, total flavonoid content, DPPH and ABTS free radical scavenging capacities and Fe3+ reducing capacity as the analysis indicators, the antioxidant activity was evaluated. In addition, analysis methods such as correlation analysis, principal component analysis and partial least squares discriminant analysis were used to explore the amino acid composition of Pleurotus ostreatus. The results showed that adding grape seed powder and artichoke leaf powder to the culture medium led to higher contents of total phenolics and total flavonoids and higher antioxidant activity compared with those of the CK group, especially the component number and content of amino acids increased significantly. There were relatively more high amino acid contributors, and the first two principal components accounted for 94.4% of the cumulative variance, with Ala, Tyr, Lys, Ser, Thr, Asp, Ary, Glu, Phe and His being the high contributors to A1 sample, Ile, Val, Gly, Pro and Leu being the high contributors to A2 sample, and no amino acid as the high contributor in the CK group. Therefore, grape seeds and artichoke leaves can be used to replace the traditional cottonseed hull culture medium to cultivate Pleurotus ostreatus, which is conducive to the enrichment of nutrients and the improvement of active components of Pleurotus ostreatus. These results provide a theoretical basis for the comprehensive utilization of industrial by-products, and have certain practical application value and significance.
