二苯乙烯化合物的提取工艺优化及其酪氨酸酶抑制活性

Optimization of the Extraction Process of Stilbene Compounds and Their Tyrosinase Inhibitory Activity

摘要:

该研究探讨了桑根来源的三种二苯乙烯化合物（氧化白藜芦醇，白藜芦醇和白藜芦醇苷）的富集提取工艺，并对提取物的抗氧化活性和酪氨酸酶抑制作用进行了研究。结果表明，通过单因素试验和响应面试验确定富集二苯乙烯化合物的最佳条件为：提取时间1 h，料液比1:20，乙醇浓度90%，在此条件下，桑根中氧化白藜芦醇，白藜芦醇和白藜芦醇苷的含量分别为2923.59 μg/g，32.45 μg/g和3.15 μg/g，富含三种二苯乙烯化合物提取物的得率为10.23%。并且，提取物表现出极强的DPPH自由基清除能力（IC50=0.063 mg/mL）和ABTS自由基清除能力（IC50=0.008 mg/mL）。此外，提取物比熊果苷表现出更强的酪氨酸酶抑制活性，其酪氨酸酶抑制率IC50（2.514 μg/mL）仅约为熊果苷（IC50=15.551 μg/mL）的16%。综上，高活性的二苯乙烯化合物提取物具有天然抗氧化剂和美白剂的潜力，在食品、美容与医药领域有很大的应用价值。桑根可作为二苯乙烯化合物的重要天然来源，是值得被开发利用的抗氧化、美白的原料。

Abstract:

In this study, the enrichment and extraction processes of three stilbene compounds (oxyresveratrol, resveratrol and resveratrol glycosides) from the mulberry root were investigated, and the antioxidant activity and tyrosinase inhibition of the extracts were also studied. The results showed that the optimal conditions for enriching stilbene compounds were determined by single factor test and response surface tests as follows: extraction time, 1 h; solid-liquid ratio, 1:25; ethanol concentration, 90%. Under these conditions, the contents of oxyresveratrol, resveratrol and resveratrol glycosides in mulberry root were 2923.59 μg/g, 32.45 μg/g and 3.15 μg/g, respectively, and the yield of the extract rich in three stilbene compounds was 10.23%. In addition, the extract showed a strong DPPH free radical scavenging ability (IC50=0.063 mg/mL) and ABTS free radical scavenging capacity (IC50=0.008 mg/mL). Furthermore, the extract showed a stronger tyrosinase inhibitory activity than arbutin, with its tyrosinase inhibitory rate IC50 (2.514 μg/mL) being only 16% of that of arbutin (IC50=15.551 μg/mL). In summary, the highly active stilbene extract has the potential as a natural antioxidant and whitening agent, and has great application value in the fields of food, beauty and medicine. Mulberry root can be used as an important natural source of stilbene compounds, which is worth to be developed and utilized as a raw material for anti-oxidation and whitening.