斗米虫蛋白对4T1荷瘤小鼠的抗肿瘤作用

In-vivo Anti-tumor Effects of the Proteins of Apriona swainsoni Larvae on 4T1 Tumor-bearing Mice

摘要:

该研究探讨了斗米虫蛋白对乳腺癌（4T1）荷瘤小鼠的体内抗肿瘤作用。通过构建BALB/c-4T1荷瘤小鼠模型，观测实验期内各组荷瘤小鼠体质量及肿瘤生长的变化，利用活体生物发光成像仪观察各组小鼠体内4T1肿瘤细胞生长情况，给药结束后处死小鼠，计算抑瘤率和各主要脏器指数。结果显示：与模型对照组相比，斗米虫蛋白各剂量组荷瘤小鼠体重无显著变化，主要脏器质量和脏器指数也无统计学差异性，但肿瘤质量显著减小（p<0.05），斗米虫蛋白低、中、高剂量组抑瘤率分别为23.08%、36.06%、21.63%。经活体生物发光成像仪检测，斗米虫蛋白各剂量组4T1肿瘤细胞的荧光信号相对模型对照组减弱，与肿瘤生长情况相一致。实验表明斗米虫蛋白具备体内抗肿瘤活性且对4T1荷瘤小鼠机体及各主要脏器无明显毒副作用。

Abstract:

4T1 tumor-bearing mice models were established to examine the in-vivo anti-tumor effects of proteins extracted from Apriona swainsoni larvae. Changes in the body weight and tumor growth of the mice treated with different protein concentrations were observed during the experiments. The growth of the 4T1 tumor cells was detected by bioluminescence imaging. All mice were sacrificed after treatment administration, and the tumor inhibition rate and indexes of main organs were subsequently calculated. Compared with the tumor model control group, the tumor-bearing mice treated with different concentrations of proteins of Apriona swainsoni larvae showed no significant changes in their body weights or the weights and indexes of main organs, whereas the tumor weights significantly reduce (p<0.05). The tumor inhibition rates in the groups treated with the Apriona swainsoni larval protein at low, medium, and high doses were 23.08%, 36.06%, and 21.63%, respectively. The fluorescence signals of the 4T1 tumor cells in these three treatment groups were attenuated in comparison with those of the control group. The result was consistent with the tumor growth observations. It was preliminarily concluded that the proteins of Apriona swainsoni larvae have certain in-vivo anti-tumor effects, and they have no obvious toxic and side effects on the body and the main organs of the 4T1 tumor-bearing mice.