本研究探讨了复方蜂胶提取物对免疫低下模型小鼠免疫功能的影响。利用氢化可的松（HY）构建小鼠免疫低下模型，设置阴性对照组、模型组、蜂胶提取物组（0.125 g/kg）、3个复方剂量组（0.125、0.50、1.25 g/kg），对小鼠连续灌胃30 d，检测各种免疫功能等指标，探究复方蜂胶提取物对小鼠免疫功能的影响。实验发现单一蜂胶提取物组和各剂量的复方蜂胶提取物均可以抵制HY对小鼠脾脏、胸腺的损伤，将脏器指数分别从2.80恢复到3.89、3.81、3.92和4.11（p<0.01）；各实验组均可显著改善HY导致的小鼠外周血白细胞（PBWC）和淋巴细胞（PBL）水平降低（p<0.05），其中高剂量组可将PBWC和PBL水平从3.62×109/L和2.83×109/L升高到5.35×109/L和4.96×109/L（p<0.01）；中、高剂量组可提高免疫低下小鼠的碳廓清吞噬指数，将其从4.16提高到5.29和5.16（p<0.01），低剂量组和单一蜂胶提取物组效果不明显；中剂量组可提高免疫低下小鼠腹腔巨噬细胞吞噬荧光微球的吞噬百分率，将其从18.70%提高到28.60%（p<0.01）；中、高剂量组可显著提高抗体生成细胞数（p<0.05）；从而表明复方蜂胶提取物具有增强免疫低下小鼠免疫力的功效。

To study the effects of propolis extract mixtures on the immune function of immunocompromised mice models constructed using hydrocortisone (HY), experiments were conducted on six groups of mice models, namely, the negative control, model, propolis extract-treated (at a dose of 0.125 g/kg), and three propolis extract mixture-treated groups (at doses of 0.125 g/kg, 0.50 g/kg, and 1.25 g/kg, respectively). Oral administrations were continued for 30 days, and the immune indexes of the mice from the different groups were determined. Results showed that the propolis extract alone and the extract mixtures at different doses can resist HY damage to the spleen and thymus of mice, restoring the organ index from 2.80 to 3.89, 3.81, 3.92, and 4.11, respectively (p<0.01). For mice from all the treatment groups, the decrease in the peripheral white blood cell (PBWC) and lymphocyte (PBL) counts induced by HY was significantly improved (p<0.05). More specifically, for the high-dose group, the PBWC and PBL counts in the immunosuppressed mice increased from 3.62×109/L and 2.83×109/L to 5.35×109/L and 4.96×109/L, respectively (p<0.01). The carbon clearance indexes of the immunocompromised mice in the medium- and high-dose groups increased from 4.16 to 5.29 and 5.16, respectively (p<0.01); however, the effects on the low-dose group and the group treated with propolis extract were not as noticeable. The phagocytosis percentage of fluorescent microspheres by peritoneal macrophages in the medium-dose group increased from 18.70% to 28.60% (p<0.01). Furthermore, for the medium- and high-dose groups, the numbers of antibody-producing cells increased (p<0.05). These results demonstrate that the propolis extract mixtures can enhance the immunity of immunocompromised mice.

山东省科学院青年基金项目（2018QN0029）