绿菇多糖的提取工艺优化

Optimization of Extraction Process of Russula virescens Polysaccharide

摘要:

该文研究了绿菇多糖的超声波辅助提取方法。运用超声波辅助浸提、乙醇沉淀、Sevage法脱蛋白等步骤提取绿菇多糖，采用苯酚-硫酸法测定多糖含量。通过单因素实验考察超声功率、温度、超声时间和水料比等4个因素对多糖得率的影响，在此基础上采用Box-Benhnken响应面法设计四因素三水平试验，建立回归方程，研究各因素对绿菇多糖得率影响的程度，进一步优化提取工艺，得到了绿菇多糖超声波辅助法的最佳提取条件为超声功率500 W、温度76 ℃、超声时间40 min和水料比31 mL/g，多糖的得率为6.50%。因此，超声波辅助法有助于提高绿菇多糖的得率，响应面法建立的回归模型及相关参数的实际值与预测值之间的相关度较好，可以用于对超声波提取绿菇多糖进行分析和预测。

Abstract:

The ultrasonic-assisted extraction method of Russula virescens polysaccharide was studied. Several steps were used to extract Russula virescens polysaccharides including ultrasonic-assisted extraction, ethanol precipitation, Sevage deproteinization, then polysaccharide content was determined by phenol-sulfuric acid method. In this paper, single factor experiments were used to investigate the influence of four factors, including ultrasonic power, temperature, ultrasonic time and water-to-material ratio, on the yield of polysaccharides. On this basis, Box-Benhnken response surface method was used to design a four-factor three-level experiment, and a regression equation was established to study the degree of influence of each factor on the yield of Russula virescens polysaccharides. The extraction process was further optimized and the optimal extraction conditions for Russula virescens polysaccharides were obtained. The optimal extraction conditions were ultrasonic power 500 W, temperature 76 ℃, ultrasonic time 40 min, and water-to-material ratio 31 mL/g. The yield rate of polysaccharide was 6.50%. Therefore, the ultrasonic-assisted method is helpful to improve the extraction rate of Russula virescens polysaccharide. The regression model established by response surface method and the correlation between the actual value and the predicted value of related parameters are better, which can be used to analyze and predict the ultrasonic extraction of Russula virescens polysaccharides.