肉桂多酚清除自由基及抑制α-葡萄糖苷酶活性的能力

The Abilities of Cinnamon Polyphenols to Scavenge Free Radicals and Inhibit α-Glucosidase

摘要:

本研究主旨是探讨肉桂多酚的体外抗氧化和α-葡萄糖苷酶抑制能力，为肉桂多酚的开发利用提供理论基础。通过福林酚法测定肉桂多酚的总酚含量，采用试剂盒以及有机试剂检测肉桂多酚清除ABTS+、Fe3+、DPPH、OH-等自由基的能力以及对α-葡萄糖苷酶活性的抑制效果。结果表明，肉桂多酚的含量为64.43 mg/g（干重），对ABTS+、Fe3+、DPPH、OH-等自由基具有明显的清除能力，其IC50值（半抑制率浓度）分别为0.71、0.27、0.18、0.97 mg/mL，对以上四种自由基的清除能力由强到弱依次为DPPH>Fe3+>ABTS+>OH-，且存在剂量依赖性，自由基的清除能力随着样品质量浓度的增加而提高。肉桂多酚对α-葡萄糖苷酶具有显著的抑制能力，其IC50值为0.048 mg/mL，同样存在剂量依赖性，且抑制效果显著高于阿卡波糖对照。因此，肉桂多酚对ABTS+、Fe3+、DPPH、OH-等自由基具有明显的清除能力，对α-葡萄糖苷酶具有显著的抑制能力。

Abstract:

The main aim of this study to investigate the in vitro antioxidant activity and ability to inhibit α-glucosidase of the cinnamon polyphenols, to provide theoretical basis for their development and applications. The total phenolic contents of the cinnamon polyphenols were determined by the folin-phenol method. The ABTS+, Fe3+, DPPH? and OH- scavenging abilities and the inhibition of α-glucosidase activity of the cinnamon polyphenols were detected using the kits and organic reagents. The results showed that the content of cinnamon polyphenols was 64.43 mg/g (dry weight), and significant scavenging abilities towards ABTS+, Fe3+, DPPH? and OH- were detected with the IC50 values (half inhibitory concentration) being 0.71, 0.27, 0.18 and 0.97 mg/mL, respectively). The scavenging abilities of the cinnamon polyphenols towards the above-mentioned free radicals were in the order of DPPH?>Fe3+>ABTS+> OH-, and exhibited in a dose-dependent manner (scavenging ability increased with an elevated mass concentration). The cinnamon polyphenols had a significant inhibitory effect on α-glucosidase in a dose-dependent manner, with an IC50 value as 0.048 mg/mL, and such inhibition was significantly greater than that of acarbose. Therefore, the cinnamon polyphenols possess significant abilities to scavenge ABTS+, Fe3+, DPPH?, OH- radicals, and inhibit the activity of α-glucosidase.