基于气相色谱-质谱法非靶向代谢谱分析，分离和鉴定了来自三个产地（北安龙门、尾山和引龙河）的黑河43号大豆的代谢产物。采用80%甲醇提取大豆样品中代谢产物，N,O-双(三甲基甲硅烷基)三氟乙酰胺进行衍生，HP-5ms柱进行分析，GC-MS进行检测，通过KEGG注释分析差异代谢物的代谢途径，并对代谢机制进行阐述。在样品中共检测到68种代谢产物，分离鉴定出62种代谢产物，包括22种糖类及其衍生物，14种脂肪酸及其衍生物，7种醇类，4种酯类，2种氨基酸和13种中间体，推断出6种未知代谢产物结构，WS-soy样品4种，YLH-soy样品2种。不同产地大豆代谢产物和代谢机制存在差异，带有产地属性。这不仅为寒地大豆品质分析提供理论基础，也为大豆的分类加工或不同产地提取功能性成分提供依据。

Metabolites of Heihe 43 soybeans in three origins (Beian Longmen, Weishan and Yinlong River) were isolated and identified using non-targeted metabolic profiling based on gas chromatography-mass spectrometry. The metabolites in the soybean samples were extracted by 80% methanol, derivatized with N,O-bis (trimethylsilyl) trifluoroacetamide, eluted on an HP-5ms column, detected with GC-MS. The metabolic pathways of the differential metabolites were analyzed by KEGG annotation, the metabolic mechanisms were explored. A total of 68 metabolites were detected in soybean samples, and 62 metabolites were isolated and identified, included 22 carbohydrates and their derivatives, 14 fatty acids and their derivatives, 7 alcohols, 4 esters, and 2 amino acids, and 13 kinds of intermediates, inferring the structure of 6 kinds of unknown metabolites, 4 kinds of WS-soy samples, 2 kinds of YLH-soy samples. Soybean metabolites and metabolic mechanisms were different in different producing areas, and they had the attributes of producing areas. This not only provides a theoretical basis for the quality analysis of soybeans in cold regions, and also provides a basis for soybean classification and processing or extraction of functional components by origin.

黑龙江省应用技术研究与开发计划重大项目（GA18B102）；黑龙江八一农垦大学三横三纵资助项目（ZRCQC201906）；黑龙江省农垦总局重点研发项目（HKKY190407）