为了研究河豚毒素（TTX）在暗纹东方鲀体内的转移分布规律及其对肝脏代谢酶活性的影响，实验选择口服灌喂的给药方式，通过超高效液相色谱串联质谱法测定不同组织中TTX的浓度变化，同时测定肝脏代谢酶活力。结果如下，在灌喂TTX后的短期转移分布期间，性腺中TTX的浓度从19.00 ng/g升至42.02 ng/g，蓄积率为121%。肝脏中TTX浓度呈波动性变化，在4 h时，TTX浓度最高为11.43 ng/g，在48 h时，TTX浓度最低为1.27 ng/mL。皮肤、肌肉、血液、肠道、鳃组织中TTX含量在2 h时最高，分别为7.43 ng/g、1.15 ng/g、11.44 ng/g、9.77 ng/g、31.75 ng/g。脾脏、肾脏、胆组织中TTX浓度分别在2 h、4 h、6 h时最高为5.05 ng/g、6.54 ng/g、9.06 ng/g。此外，肝脏代谢酶活力的检测结果显示，TTX显著提高了乳酸脱氢酶、谷草转氨酶和谷丙转氨酶的活性（p<0.05），在前期对两种磷酸酶活性具有明显的抑制效应（p<0.05），而后恢复至正常水平。实验结果表明，TTX在暗纹东方鲀中的转移分布具有组织特异性，并且对肝脏中不同代谢酶活性具有促进和抑制作用。

In order to study the transfer and distribution of tetrodotoxin (TTX) in Takifugu obscurus and its activity on liver metabolizing enzymes, the oral gavage administration method was used in the experiment. The concentration changes of TTX in different tissue were measured by ultra performance liquid chromatography tandem mass spectrometry, and the activity of metabolic enzymes in the liver was also measured. The results were as follows, during the short-term metastasis distribution period after TTX instillation, the concentration of TTX in the gonads increased from 19.00 ng/g to 42.02 ng/g, and the accumulation rate was 121%. The TTX concentration was fluctuated in the liver. At 4 h, the highest TTX concentration was 11.43 ng/g, at 48 h, the lowest TTX concentration was 1.27 ng/mL. The TTX contents in the tissues of skin, muscle, blood, intestine, and gills were highest at 2 h, which were 7.43 ng/g, 1.15 ng/g, 11.44 ng/g, 9.77 ng/g, and 31.75 ng/g, respectively. The highest TTX concentrations in the spleen, kidney and gallbladder tissues were 5.05 ng/g, 6.54 ng/g, and 9.06 ng/g at 2 h, 4 h, and 6 h, respectively. In addition, the test results of liver metabolic enzyme activity showed that TTX significantly increased the activities of lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase (p<0.05). And it had a significant inhibitory effect on two phosphatase activities in the early stage (p<0.05), and then returned to normal levels. Theresults indicate thatthe transfer and distribution of TTX in Takifugu obscurus has tissue specificity, and can promote and inhibit the activities of different metabolic enzymes in the liver.

国家重点研发计划项目（2017YFC1600701）